Effects Of Arsenic Trioxide On Migration And Adhesion Of Osteosarcoma Cell Line MG-63

Objective:To study the effects of arsenic trioxide on migration and adhesion of osteosarcoma cell line MG-63.Methods:the cell line MG-63 was regulated cultured. Three groups were divided as follows:experimental group interacting with arsenic trioxide( 1.0μmol/L) for 48 hours, positive control group processed with 5-Fu(15mg/L), negative control group dealt with cell culture medium of the same volume. Cell aggregation assay and Laminin adhesion assay were utilized to investigate its homogeneous and heterogeneous adhesion abilities;scarification test and transwell assay were employed to detect its migration ability.P<0.05 was statistically significant, according to statistics for paired or independent sample t test.Results:after being treated with arsenic trioxide, cell aggregation assay demonstrated that aggregation index of experimental group with incubation time for 20 min,40 min, 60 min was 0.24 ± 0.030, 0.47 ± 0.059 and 0.59 ± 0.070, significantly higher than that of negative control group(0.14±0.011, 0.18±0.015, 0.22±0.023) or positive control group(0.16±0.012,0.20±0.024,0.33±0.23), P<0.05, while Ln adhesion assay found a weaker ability of cell adhesion with incubation time for 60 min, 90 min to Ln in experimental group(0.144±0.007,0.141±0.013) than that in negative control group(0.387±0.026, 0.461±0.024) or positive group(0.363±0.035, 0.513±0.034), P<0.05;scarification test indicated that cell migration distance, area and rate of the experimental group(192.3±16.7, 91.9±9.1, 4.00±0.35) were lower than that of negative(362.0±27.8, 242.5±21.2, 8.2±1.1) and positive(301.6±23.8, 161.9±12.9, 6.9±0.9) control group. Transwell assay demonstrated that transmembrane cells in experimental group、positive group and negative control group respectively were(112±11)、(172±15)、( 276 ± 37), P<0.05. Conclusion: arsenic trioxide in vitro promotes homogeneous adhesion of osteosarcoma cell line MG-63 yet inhibits heterogeneous adhesion and cell migration.