| Objective:Based on the neurotrophic hypothesis of depression.by observing the behavioral changes of depression model rats with chronic unpredictable mild stress(CUMS) and after escitalopram intervention and meaturing the level of brain-derived neutrophic factor(BDNF) in cerebrospinal fluid(CSF) and serum with ELIS A,this study was aimed to investigate the relationship between BDNF and depression and to explore the effects of escitalopram on BDNF.Methods:The depression rat model was established with chronic unpredictable mild stress.The depressed behaviors were observed through forced swimming test(FST),sucrose preference test(SPT),open-Field test(OFT) and body weight growth.The level of BDNF in CSF and serum was meatured by ELIS A.Results:1. After 4 weeks of CUMS,compared with control rats, immobility time in FST was significantly prolonged [(15.18±8.39)s vs. (73.33±34.71)s];sucrose preference percent in SFT was significantly decresed [(86.21±5.54)% vs. (69.68±14.29)%];locomotor distance in OFT was significantly decresed [(1328.65± 513.29)cm vs. (375.71±176.18)cm],;rearing activity in OFT was significantly decresed[(19.58±6.90) vs. (3.25±3.25)] and body weight growth was significantly slow[(376.04±27.27)g vs. (318.97±34.290)g] in CUMS rats. The differences were all statistically significant(p<0.01).2.After 4 weeks of escitalopram intervention, compared with depression rats, immobility time in FST was significantly decreased[(59.50±31.06)s vs. (20.41± 10.67)s]; sucrose preference percent in SFT was significantly incresed[(48.81± 18.90)% vs. (85.27±7.76)%]; locomotor distance in OFT was significantly incresed[(267.76±255.56)cm vs. (1190.41±306.64)cm] in depression+escitalopram rats.The differences were all statistically significant(p<0.05). There were no significant differences in both rearing activity in OFT[(5.17±3.71) vs. (11.17±1.33)] and body weight[(417.03±43.82) g vs. (437.30±57.24)g] between depression and depression+escitalopram rats(p>0.05).3. After 4 weeks of escitalopram intervention,there were no significant defferences in immobility time in FST[(14.00±6.60)s vs. (18.39±5.37) s], sucrose preference percent in SFT[(90.19±4.28)% vs. (94.08±2.66)%], locomotor distance[(1575.06±471.96)cm vs. (1373.38±316.30) cm] and rearing activity in OFT [(14.17±4.45) vs. (17.67±1.67)]and body weight[(437.30±57.24)g vs. (417.03±43.82) g] between control+escitalopram and contronl rats(p>0.05)4. BDNF in CSF:Compared with control rats,the level of BDNF in CSF was significantly decreased in depression rats[(1306.30±170.15)pg/ml vs. (1064.69 ±97.31) pg/ml].Compared with depression rats,the level of BDNF in CSF was significantly increased in depression+escitalopram rats[(1064.69±97.31) pg/ml vs. (1320.36±189.23) pg/ml].The differences were both statistically significant (p<0.05). There was no significant difference in the level of BDNF in CSF between control rats and control+escitalopram rats[(1306.30±170.15)pg/ml vs. (1416.32±73.48) pg/ml] (p>0.05).5. BDNF in serum:Compared with control rats,the level of BDNF in serum was significantly decreased in depression rats[(688.14±68.87)pg/ml vs. (374.16± 166.73) pg/ml]. Compared with depression rats,the level of BDNF in serum was significantly increased in depression+escitalopram rats[(374.16±166.73) pg/ml vs. (844.17±79.57) pg/ml].The differences were both statistically significant (p<0.01). There was no significant difference in the level of BDNF in serum between control rats and control+escitalopram rats[(688.14±68.87)pg/ml vs. (544.90±97.35) pg/ml] (p>0.05).Conclusions:The level of BDNF in both cerebrospinal fluid and serum of depression model rats is decreased. Escitalopram intervention can improve depressive behaviors and increase the level of BDNF in both cerebrospinal fluid and serum, but has no effects on normal rats. |
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