Potato Topical Ointment For Traumatic Synovitis Of Knee Joint Fluid In Rabbit IL- 1β, TNF- ɑ And Serum SOD, MDA Influencemechanism Research

Objective By observing the "potato topical ointment"(Gansu university of traditional Chinese medicine hospital massage recovery since the quasi square, formula is: potatoes + borneol + alum) model of rabbit traumatic synovitis of knee joint synovial structure change and SOD and MDA in serum and joint fluid of IL- 1 beta, influence of TNF- ɑ, to explore traditional Chinese medicine "potato topical ointment" the curative effect of traumatic synovitis of knee joint intervention.Methods Experimental animals to choose New Zealand white rabbit only 40, male and female half, 3-4 months, weight 2.2 2.8 kg, it will first randomly divided into 2 groups: group A blank(10), another group of B module, made after the success of the mould and then B module is divided into 3 groups, respectively is: C model control group(only build mould do not make any treatment), D helped him Lin emulsion agent in the control group(after the success of the model making external intervention helped him Lin emulsion agent 5 days), E potato mixture in treatment group(after the success of the model making external potato mixture intervention 5 days), each group of 10 New Zealand white rabbit, only A single use on the right side of the knee joint, A total of 40 joints. Adaptability to feed a week later, the New Zealand white rabbit fixed, with 8% of the sodium sulphide shed hair around the right hind leg knee joint, press with 3% pentobarbital sodium LML/kg by ear marginal vein slowly push note anesthesia(time strict control in no less than 2 min), vertical with acupuncture needle pierced each New Zealand white rabbit right hind leg quadriceps and gastrocnemius midpoint, connect a "great" KWD 808 a type I pulse acupuncture therapeutic apparatus, electricity, the use of continuous wave form, frequency was 50 hz, the intensity of fixed in 6.0 mv(suitable for building the animal’s resistance strength, according to preliminary experimental determination: if the strength is less than 6.0 mv, rabbit knee flexion range is not big, cannot achieve the strength of the model; if the strength is greater than 6.0 mv easily lead to avoid muscle spasms, make joint spasticity, thus affecting model), the building animal knee keeps the knee joint synovial damage passive flexion and extension, 2 times a day, each time 45 min, to 10 days in a row, establishment of animal model. Building after the start of each day is generally measured rabbit building limb knee weeks diameter change, articular surface temperature, and so on and so forth. Making potato mixture(gansu university hospital of traditional Chinese medicine massage recovery from party) : clean the potato, beaten clay, sprinkle evenly by certain proportion of alum and borneol(the specific proportion is: potato: alum: borneol = 100:4:1), cold storage(make a potato mixture for two days at most dose); Animal drug delivery: after model establishment, D he Lin of emulsion agent control building knee external intervention helped him Lin emulsion agent, at about 4 mm thickness covering the surface of the joints, 2 times a day, lo intervention treatment for five days. The potato topical ointment topical E potato mixture group a rabbit knee injuries were local, chitin, 2 times a day, continuous treatment 5 days; Chitin is completed, the New Zealand white rabbit femoral artery blood, take on centrifugal clear liquid(centrifugal concrete for operating conditions: 3500 r/min, 10 min), and the serum in centrifugal income- 20 ℃ cryopreservation inactive; New Zealand white rabbit executed by air embolism, executed with a scalpel to open a rabbit were right knee joint capsule, after general observation records with LML saline lavage joint cavity, strict careful joint fluid collection, put in has silicide in vitro, centrifugal(centrifugal concrete for operating conditions: 4 ℃, 1800 r, 10 min), puts the supernatant- 70 ℃ cold storage. Take a knee joint synovial specimens were, and placed in 4% paraformaldehyde fixed. Will make routine pathological synovial HE staining, the observation of the synovial membrane structure change. The results detected by enzyme-linked immunosorbent(ELISA) detection of interleukin 1(IL- 1 beta), tumor necrosis factor(TNF- ɑ), using the method of xanthine oxidase(hydroxylamine hydrochloride method) to detect the contents of superoxide dismutase(SOD), using glucosinolates barbiturate(associates) method to detect malondialdehyde(MDA) content. Using spss18.0 software for statistical analysis of experimental data.Results 1. After building, white rabbit model of traumatic synovitis of knee joint group in general, compared with the blank group, the knee joint with obvious swelling, synovial thickening, joint surface temperature increase obviously(P < 0.01). 2. Before the potato topical ointment intervention treatment, building the big white rabbit compared with the normal white rabbit, the joint fluid of TNF alpha 、beta IL- 1 and MDA content increased significantly, SOD content significantly decreased(P < 0.01); After the intervention, the potato topical ointment group white rabbit serum TNF alpha and beta IL- 1 content is significantly lower than the model group, SOD and MDA content is higher than that of model group(P < 0.01), but the comparative analysis and helped him Lin emulsion agent control group, there was no statistically significant difference(P > 0.05).Conclusion 1. The rabbit traumatic synovitis of knee joint after the success of the model establishment, proinflammatory cytokines TNF-α in synovial fluid and SOD and MDA in the serum IL- 1β all have obvious changes, shows the onset of traumatic synovitis of knee joint in proinflammatory cytokines are closely related. 2. The potato topical ointment can effectively inhibit inflammatory factors the expression of TNF-α and beta IL- 1β, enhance SOD,The content of MDA, reveals the potato topical ointment at the molecular level for the curative effect of traumatic synovitis of knee joint.