Study On Correlation Between The Ultrastructural Changes Of The Brain Tissue Surrounding Hematoma After Intracerebral Hemorrhage And The Excess Syndrome Of Stroke And Prostration Syndrome In Intracerebral Hemorrhage Patients

Objectives:This study draws the material from the surrounding of hematoma which look directly in operation.To observe the brain tissue’s ultrastructure change at the different type of syndrome which surrounding hematoma,and the volume of blood tumor,scores of NIHSS and GCS.Probing the possible mechanisms of the secondary injury after intracerebral hemorrhage,providing the objective indicator and molecular cytobiology standard for the TCM syndrome differentiation,offering the more guidance for the clinical medication and hope study,and providing a new theory direction for the Combination of TCM and Western medicine. Materials and methods:30 patients with ICH collected which 16 were male and 14 were female,and divided into three groups:YIN excess group(8 were male and 6were female), YANG excess group (4 were male and 5were female) and Prostration group (4 were male and 3 were female).For GCS and NIHSS score, calculation of the amount of bleeding under the DUO TIAN formula. All the specimen specimen were picked macroscopic under the surgical operation,and made strictly by the rule,and observed by two associate professor who full of experience.Results:1.Ultrastructure:The tissue’ organ of Yang group were almost normal.Part of cells were swelling,the cell-organelle reduced,and also mitochondrial cristae. Myelin sheath were vague, neurofilaments were generally normal.Capillaries were generally normal,but pinocytotic vesicles were rased in endothelial cells. In the Yin group,cells were swelling obviously, cell crgan reduced clearly, endochylema and mitochondria changed like vacuole,part of cells necrosis and disintegrated,and a lage number of autophagic vacuoles appeard,nucelus was pyknosis. Myelin sheath was edematous,part of the myelin sheath layers disrupt.The diameter of capillary became narrow by the swelling of the capillary endothelial cells.In the Prostration group,a lage sheet of brain tissues were necrotic,some of them were desolateness,cell membrane incomplete, nuclear membrane was concave,intranuclear chromatin agglutinated,and nucleolus disappeared.The capillary lumen collapsed,the tight junction was broken. The myelin sheath layers disrupt and disintegrated,part of the content of neuraxis was dissolving.2.The ultrastructure damage,the hematoma volume,the scores of NIHSS in each three groups.compaired with each two,the sequence like this:Prostration Syndrome> YIN Excess Syndrome>YANG Excess Syndrome,the GCS,and the GCS is contrary.Conclusion:1. Ultrastructure:The tissue’ organ of Yang group were almost normal,part of cells were cytotoxic edema.In the Yin group,cells were swelling obviously, endochylema and mitochondria changed like vacuole,a lage number of autophagic vacuoles appeard,nucelus was pyknosis. Myelin sheath was edematous.The diameter of capillary became narrow by the swelling of the capillary endothelial cells.In the Prostration group,a lage sheet of brain tissues were necrotic,cell membrane incomplete, nuclear membrane was concave,and nucleolus disappeared.The capillary lumen collapsed,the tight junction was broken. The myelin sheath layers disrupt and disintegrated,part of the content of neuraxis was dissolving.2.The hematoma volume,the scores of NIHSS and GCS can be taken as the objective indicator and molecular cytobiology when we standard for the TCM syndrome differentiation.Also we can figure out that the ultrastructure damage is probablely positive correlation to the hematoma volume and the scores of NIHSS,but negative correlation to the scores of GCS.