The Analysis For Cell DNA Damage Products 8-OH-dG And Antioxidants Protection With Capillary Electrophoresis

DNA, as a genetic material, is easy to be damaged in cells. The oxidative damage is the main DNA damage type. The imbalance between oxidation and anti-oxidation in the body can lead to oxidative stress, meanwhile, accumulation of free radicals easily lead to cell damage,further induce the occurrence of disease and aging. Thus it is especially important to find a sensitive method to detect DNA oxidative damage. The structure of DNA made it complexity and difficulty to detection, so detective method of of DNA oxidative damage markers becomes a hot research topic. Papers reported that there are about 20 markers of oxidative damage of DNA, 8-OH-dG is easily to check out, was unanimously considered to be one of the important markers of DNA oxidative damage. Furthermore, capillary electrophoresis quickly become a new kind of analysis technology in analytical chemistry field. The rapidition and effective of capillary electrophoresis make it quickly become a hot theme in food, medicine, forensic, environment, biology science and other fields.This research dedicated to investigate new separation detection technique, capillary electrophoresis detect the 8-OH-dG of cells treated with different reagents. Finally, achieve the purpose of detecting toxicity. And treated cells with different antioxidants stresses, then detecting the 8-OH-dG so that response the antioxidant. This paper aims to build a new and effective antioxidant activity of detecting methods. The results are as follows:(1) Separation conditions as follows: temperature: 20 ?; Voltage: 20 kv; 10 mmol/L borate buffer, pH 9.0; 20 s hydrodynamic injection. Detection time: 10 min; The sample solvent: three steamed water; Non Dynamic pH Junction.(2) Separation conditions of detection 8-OH-dG of HL-60 as follows: temperature:20 ?;Voltage: 20 kv; 10 mmol/L borate buffer, pH 9.0; 20 s hydrodynamic injection.Detection time: 10 min; The sample solvent: three steamed water; Non Dynamic pH Junction.At the same time optimizing the key steps of hydrolyzed DNA. In addition HL-60, as H2O2 concentration increasing, the greater the oxidative damage of DNA.(3) CE detection of different antioxidants after it protect HL-60, the results showed that after lycopene, quercetin and curcumin protect HL-60 the DNA damage have increased with caused by H2O2.(4) When the U87 treated with rotenone to set up Parkinson's disease model. CE detect8-OH-dG of cells, the results show that rotenone cause obvious U87 cells damage. After different antioxidant treat with cells and detecting, the results showed that curcumin and quercetin have a protection effect on rotenone damage.