Identification Of A Micro-Algae And Its Culture Conditons For High Production Of Starch And Total Sugar

Non-renewable resources such as fossil energy content is limited and polluting the environment, energy issues have become key issues constraining human development, more and more people is needed to find clean energy sources. Microalgae is easy to train and fast growth which no pollution can be used for biodiesel and bioethanol, it has been widely studied in recent years. Other microalgae Chlorella cells contain large amounts of starch, polysaccharides, cellulose (la type), these substances through a simple process, in accordance with conventional technology to ethanol into fuel ethanol. Moreover, microalgae can be tolerated industrial emissions, making it beneficial for the conversion of carbohydrates, proteins, fats and other substances. Improve the carbohydrate content of starch and other microalgae for post-fermentation can significantly improve the conversion efficiency and reduce costs.In this paper, four microalgae:Scenedesmus #3, green algae CZ, green chlorella SO, Scenedesmus PM2, by comparing these four microalgae biomass production rate, starch content and total sugar content, select microalgae #3 which has high biomass and high starch for further study. Identified by 18S rDNA analysis, etc., microalgae #3 have been identified as Desmodesmus sp. Analysis by HPLC microalgae #3 of total sugar, xylose, glucose, indicating that the growth of microalgae #3, the wavy glucose levels fluctuate. By N source, P source, S source regulation of microalgae #3 optimized culture conditions and measuring the growth of algae and algal cells, starch and sugar content. Lack of N condition the highest biomass of microalgae is 0.98 g/L, and under normal conditions is 3.6 g/L. Total sugar and starch content representing55.4% and 21.1% of the dry weight in 2nd days, respectively, in favor of the accumulation of sugars. N concentrations under various other conditions, the biomass gap is not obvious, at 2 mg/L NaNO3 conditions, the total sugar content to reach the peak, accounting for 31.3% of dry weight in 3th days, dry weight of only the highest starch content of 7.1%. Lack of P and P-rich conditions, are conducive to the accumulation of sugars microalgae, but for algae growth and not much help.Lack of P and P-rich conditions, the highest biomass were 1.05 g/L and 2.9 g/L, but under normal conditions is 9.68 g/L. In the absence P conditions, the total sugar content of microalgae rising after inoculation, in 10 days reached accounted for 41.9% of the dry weight of the starch content is peaked, accounting for 22.9% of dry weight in the 7th days. At 0.02 g/L P concentration, total sugar and starch content reached the highest value in the 4th days, accounting for 30% of the dry weight and 27%, respectively. Lack of S will affect the growth of algae, but a small amount of S-rich microalgae and a little lack of growth for the S and not much affected. S under conditions of absence, the maximum biomass of microalgae to 0.79 g/L, the normal condition is 2.5 g/L. Lack of S conditions, respectively, of total sugar and starch content in the 4th and 2nd day after inoculation reached the maximum dry weight accounted for 35.8% and 29%. A small amount of missing and a few rich S under S, the total sugar content in the 7th and four days to reach the peak, accounting for 22.7% of dry weight and 32.2%, respectively, and total sugar content similar to starch content also were in the 7th and The first four days to reach the peak of 18.1% of dry weight, respectively, and 30.1%. The article also simulated exhaust laboratory, algal cells by measuring the starch and sugar content, case studies microalgae accumulated in the exhaust gas conditions starches and sugars. Indicates that the exhaust gas under the conditions of microalgae, many early enough sugar is accumulated, up to 70% of the dry weight of the microalgae, but the latter is not very conducive to the accumulation of algae polysaccharides.