Genome Multiple Alignment And Evolutionary Studies Of Solanaceae Lineages

The research object is the four genome of solananceae and two important dicotyledonous models-grape and coffee, using the research methods of comparative genomics, determining the evolutionary scale of polyploidization process, inferring the genomic structure and chromosomal evolution pattern after polyploidization, clarifying the results of polyploidy caused by evolution of each species.The homologous structure analysis of multiple genomes showed that the ancestor of the solanaceae family experienced two times whole-genome triplication events. The two whole-genome polypoidization is accompanied by a large number of genes lost, and the distribution of the missing fragments is a geometric distribution.Research has obtained the homologous genes of whole genome polypoidization, using genome homologous structure and strict collinear genetic search. Tomato genome has the most homologous chromosome segment(354) and duplicated gene pairs(4196), accounting for 12.1% of its genome. Finally, a combined comparison map was constructed based on the reference genome of grape and coffee.Synonymous nucleotide substitution rate(Ks) showed that the Ks value of tomato was highest(0.75); potato has a slowest rate of evolution in several solanaceous species with a minimum Ks value(0.65). It was found that the pepper was first differentiated in 29Mya; the eggplant was closely followed in 24Mya; the time of differentiation of tomato and potato was near in 12 Mya.The alignment information list, which including six species of orthologous and paralogous, will provides extensive and valuable resources for the research on the structure, function and evolution of the family of solanaceae.