The Single Molecule Dynamics Measurement And The Biomaterials Design Based On Fluorescence Techniques

Due to the high biocompatibility,the fluorescence always keeps highly attention in all kinds of field,including physics,biology as well as medical.Not only the fluorescence imaging but also the fluorescence materials are keeping its influence.The single molecule fluorescence imaging technologies,as a newly developed fluorescence imaging technologies has a very high accuracy.The single molecule observation can reduce the impact of molecular average which totally stand out of other observation method.On the other hand,the light emitting materials have a lot of demand,such as LED,monitors,sensors,artificial photosynthesis and solar cells.At the same time,in order to achieve high biocompatibility,the peptide self-assembled hydrogel has been choice.So,the light emitting hydrogels based on self-assembly peptide have a huge glowing prospect.In this paper,the single molecule fluorescence technique was used to study the conformational kinetics of estrogen receptor and the developed a environmental pollutant detection platform as well as fluorescence supramolecular hydrogel system.The main contents include:Chapter 1:The single molecule fluorescence is a well-known method studying the molecule dynamics and molecule-molecule interactions.However,nonspecific absorption of biomolecules to the coverslip will cause a great difficulties to the observation of the single molecule,especially the interaction systems which need high concentration of protein.In this chapter,a simple and effective method has been introduced.In particular,a simple 10-minute Tween-20 treatment on the PEGylation coverslip will improve the hydrophilicity,and the efficacy will promote 5 to 10 times.The procedure has a broad application and convenience,which might be widely used in the single molecule fluorescence studies.Chapter 2:Endocrine disorders caused by endocrine disorders are gain more and more attentions.Endocrine disruptors affect humans or wild animals by imitating or canceling the natural hormones.As the concentration of natural hormones itself is very low,the endocrine system will have a strong reaction to the trace of endocrine disruptors.Estrogen receptors as an important pathway protein is one of the interest to researchers.In this paper,we use single molecule fluorescence technique to study the conformational kinetics of estrogen receptors and proposed an environmental pollutant detection platform to achieve high sensitivity,rapid and quantitative detection of environmental pollutants.Chapter 3:Light Emitting hydrogels may have a variety of potential applications in soft electronics.However,fabrication of mechanical stable and printable white-light emitting hydrogels remains challenging.Here,we report a kind of LEH based on the co-assembly of light-emitting metal-ligand complexes with peptide hydrogelators.Because the ligands are covalently linked to the peptide hydrogelator,the metal ions are fixed to the hydrogel network at specific positions,which could avoid the aggregation and self-quenching of chromophores.Moreover,the formation of metal-ligand complexes could introduce additional interactions to stabilise hydrogel network,making the LEH even more stable after the incorporation of metal ions.Therefore,the LEH show high mechanical and chemical stability in aqueous conditions.The LEH is optically transparent but highly fluorescent when being photo-excited.The wavelength of emission light can be controlled using different excitation light.By using three different metal ions,the white light emitting supramolecular hydrogel has been achieved.As a proof-of-principle,we demonstrate the printability of the hydrogels to various patterns as light emitting devices.We anticipate that with the improved light emitting performance and stability,this kind of LEH can find broad applications in extrusion-based 3D printing for the construction of soft electronics.