Molecular Mechanism Of BAK1 And SERK1 In Regulating Arabidopsis Floral Organ Abscission

BAK1 and SERK1,members of Arabidopsis SERK subfamily,play important roles in a variety of signaling pathways to regulate growth and development.In current research,we identified that serkl-8 bakl-4 show a delayed floral organ abscission phenotype.Statistic analysic showed significant differences in the number of unabscissed floral organs among serkl-8 bakl-4,Col-0,serkl-8 and bakl-4 at stages 7,11 and 15.Scanning electron microscope analysis show irregular abscission zone(AZ)of serkl-8 bakl-4,in which sepal(Se),petal(Pt)and stamen(St)AZ can not completely differentiate.Detailed morphological studies suggested uncompleted differentiation may have caused the delayed abscission in serkl-8 bakl-4.Expression analysis revealed that both SERK1 and BAK1 are highly expressed in AZ.Therefore,it is likely that SERK1 and BAK1 are involved in regulating floral organ abscission.Yeast two hybrid analysis showed SERK1 and BAK1 directly interact with HAE.BiFC and Co-IP analyses confirmed that SERK1 and BAK1 can directly bind HAE in planta.Our results sugessted that BAK1 and SERK 1 may directly or indirectly regulates the IDA-HAE/HSL2.