Migration Characteristics Of Methanogenic Consortia In Coal Seam

Aiming to investigate the migration characteristics of methanogenic consortia in coal,the current study focused on firstly methanogenic consortia extracted and cultured from mine water were further applied to the adsorption experiments,diffusion experiments,leaching and biological metabolism simulation experiments.Meanwhile the coal is mainly collected from Qianqiu mine,Yima,Shaqu mine,Liulin,Guandi mine,Xishan and Baiyinhua mine,Inner Mongolia.Then the surface of coal were observed by scanning electron microscopy,and analysis changes of the change of coal pore structure after treatment with methanogenic consortia by microscopic CT experimental system for comprehensive analysis migration characteristics of methanogens in coal seam.The results show: a.The adsorption ratio of the coal sample to the methanogens increased with the time increasing,and the adsorption equilibrium was reached at 120 min.The smaller the particle size of the coal sample was,the more obvious the adsorption effect on the methanogens was.As the coal rank increased,the adsorption effect on the methanogens decreased first and then increased;b.The diffusion effect of methanogens in Yima coal sample is the best,and the diffusion effect of methanogens in Guandi coal sample is better than Shaqu coal sample.The larger the particle size of coal samples with the better the permeability of coal pillars is easier diffuse to bacteria and has the faster the diffusion rate;c.The larger the particle size of the coal sample,the easier methanogenic consortia to migrate with the infiltration flow.The migration of methanogenic consortia with the infiltration flow in Yima coal sample is better than that Shaqu coal sample.In the process of migration,only a small proportion of the bacteria migrate with the movement of infiltration water flow,and most of the cells are trapped in the coal seam.d.The lower coal rank coal in the process of biological methane metabolism,the smaller the coal particle size is,and the more easily degraded to produce methane.e.The diffusion of methanogens and the biological methane metabolism process of coal samples are in direct proportion to the adsorption of methanogens.So the adsorption of methanogens is an important factor affecting their migration.f.Scanning electron microscopy showed that the methanogens were mainly cocci and bacilli,and the methanogens were more likely to adsorb on the surface of coal with low coal rank.g.The coal samples can be found to be significantly expanded after the adsorption and migration of the methanogens by comparing the CT images before and after treatment with methanogenic consortia.Meanwhile,the rate of change from the coal pore indicates that the adsorption,migration and biological methane metabolism of the methanogens in the Baiyinhua and Yima coal samples of the low coal rank have better effect,while the Shaqu coal sample is poor.