| In this thesis, Fmoc-diphenylalanine peptide was chosen as model molecules toinvestigate its self-assembly behaviors in water and aniline solvent, respectively. Onone hand, we designed and prepared a novel Fmoc-FF/alginate hybrid hydrogel andgel bead using Ca2+ions as a trigger and cross-linker. The structures and stability ofthis gel were characterized by SEM, FTIR, and so on. Furthermore, docetaxel waschosen as model drug to investigate the drug release behavior from drug-loaded gelbeads in vitro. On the other hand, we investigate the self-assembly behaviors ofFmoc-FF in aniline solvent in different concentrations, temperature, and interfaces.The morphologies and structures were characterized by SEM, AFM, and so on. Theself-assembly mechanism was also analyzed in this work.(1) The alginate was mixed with Fmoc-FF solution under stirring. The resultingmixture was injected dropwise into a CaCl2solution, leading to the formation ofstable and uniform Fmoc-FF/alginate gel beads.(2) The Fmoc-FF/alginate hybrid hydrogel was composed of self-assemblednanofibers cross-linked with alginate by Ca2+ions, leading to a significantly enhancedstability compared to the Fmoc-FF hydrogel and alginate hydrogel.(3) The sustained and controlled drug release from this hybrid hydrogel beadswas achieved by varying the concentration of Fmoc-FF and alginate. With increasingalginate content in gel beads, the release rate and the cumulative amount of docetaxeldecreased.(4) Fmoc-FF can self-assembled in aniline solvent, forming a transparent gelcomposed of helical nanofibers at a high concentration (>30mg/mL). The obtainedFmoc-FF gels are responsive to temperature, and the Fmoc-FF sol–gel process isthermoreversible.(5) With the volatilization of aniline, Fmoc-FF was self-assembled into helicalnanoribbons composed of several nanofibers on the glass surface at a lowconcentration (2-10mg/mL). The structure and size of this nanoribbon depended onthe concentration of Fmoc-FF and the volatilization rate of aniline. |
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