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Construction Of Targeted Anionic Water-soluble Conjugated Nanoparticles As A Drug Carrier Delivery

Posted on:2016-10-04Degree:MasterType:Thesis
Country:ChinaCandidate:B B HongFull Text:PDF
GTID:2311330473461071Subject:Materials Science and Engineering
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Over the past decade, great progress has been achieved for organic electronic devices and bio chemical sensor based on the water-soluble conjugated polymer organic functional materials. Due to the excellent optical properties, good water solubility and biocompatibility advantages,water-soluble conjugated organic polymer materials have been widely used. In the structure, there are two properties in water-soluble conjugated organic polymer materials. First with the unique optical properties, it can be used for a variety of biochemical sensors detection, which detection a variety of soluble ions of high sensitivity fluorescence.Secondly, it has good water soluble property and biocompatibility, often used as drug or gene carrier, combined with the target molecule and the drug or gene will be specific delivery to tumor cell site. It has become a new method of diagnosis and treatment of cancer. Based on this background, a series of targeted water-soluble conjugated polymer nanoparticles were synthesized in this thesis and we study their photophysical properties, basic particle size and drug Loading and release. Besides, with the Low cell toxicity, the PF-RGD was applied in cell imaging.(1)A new targeted anionic water-soluble conjugated organic polymer nanoparticles was synthesized. The studying of contrast of photophysical properties and particle size in water indicates that the nanoparticles was successfully combined with the molecule RGD. Then, after the medicine Dox loaded on the nanoparticles, we study its drug Loading rate and drug release rate. Based on its Low cell toxicity, this nanoparticles was used for cell imaging.(2)A new targeted cationic water-soluble conjugated organic polymer nanoparticles was Synthesized. We study its photophysical properties, particle size and Zeta potential in water and come to conclusion that this nanoparticles can be stable in aqueous solution. And then, its drug loading rate and release rate was in-depth studied. The experiment indicates that drug can be loaded on this nanoparticles and achieve drug sustained release. So we test its cytotoxicity by means of MTT assay to ensure its low cytotoxicity. At last, the nanoparticles was added to the cells for incubation, and from the cell imaging by confocal laser scanning microscopy (CLSM), we can conclude that this complex nanoparticles could be taken up by cells easily.(3)A new magnetic mesoporous silica was designed and synthesized.Its TEM and SEM picture was studied and then we studied its particles size which the results show that its particles size is between 60-70nm. After that,the study of its Zeta potential indicate that the surface of this nanoparticles with Low positive charge. And then we study its particles size under different TEOS concentration. Besides, its cytotoxicity was studied which it shows low cytotoxicity. This results shows that this nanoparticles is fit for anticancer drug Loading and shows its potential applications in biomedical area.
Keywords/Search Tags:water-soluble conjugated organic polymers, targeted therapy, cell imaging
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