Aggregation And Application Of β-Lactoglobulin Amyloid Fibril

Not only associated with numerous neurodegenerative disorders, amyloid fibrils are but also an excellent nano material whose star is rising rapidly. Regulating and controlling of aggregation kinetics and properation of composite materials with inorganic structures are both important research topics about amyloid fibrils. Herein, β-lactoglobulin and it’s amyloid fibril(β-LG/β-LGF) were regarded as a mode protein to investigate the inhibition and disaggregation capabilities of couple inorganic materials with uncovering their interaction mechanism. At the same time, novel functional amyloid-inorganic nano-composite materials were prepared by rational designed and eco-friendly approach.More details are given as follows:1. Based on the knowledge of which graphene oxide(GO) can inhibit amyloid aggregation, we investigated the binding affinity between β-LG/β-LGF and GO by multi-spectroscopic methods. As a result, the effects on their interaciton mechanism from comformational change and size of GO were made clear. Hence a novel GO-β-LGF gel were self-assembled by biomimetic mineralization, which prove a theoretical basis for combination and application of amyloid nano composite materials.2. An energy transport chain sourced from laser were designed to disaggregate amyloid fibrils. The 4-(1,2,2-tris(4-aminophenyl)vinyl)benzenamine(ATPE) were employed as an energy acceptor from photon and transited onto the first excited state level. GO played an role of energy delivery from ATPE emission to β-LGF. Shortened fibrils less than 0.8 μm were obteined by taking this novel method. In addition, the mechanism of energy delivery and disaggregation were discussed.3. By adding different concentrations of chloroauric acid, two kinds of gold nanoclusters with red and yellow fluorescence were synthesized by β-LG directly in alklai condition. Hydrothermal assays of Au@β-LG showed the fact that the red one can extension the lag time and the yellow one can prevent nucleating during β-LGF formation.4. A novel functional amyloid-alloy nanoparticles composite membrane was prepared by β-LGF directly. In the assistance of copper, an oxidation-reduction cascade were designed to reduce time and cost. The catalytic activities of these porous membrane were evaluated by p-nitrophenol(4-NP) reduction. Whereupon, a wilder application of β-LGF-inorganic materials is coming up.