Research On The Toxicity Effects Of Carbamazepine On The Chlorella,Daphnia Magna And Zebrafish

The ecological effects of residual Pharmaceutical and Personal Care Products(PPCPs) in the water environment have gradually attracted more attention in recent years as the large-scale applications. PPCPs are emerging as environmental contaminants. However, studies on the toxic effects of PPCPs on aquatic organisms have not been adequately researched.In the present paper, three kinds of aquatic organisms(Chlorella, Daphnia magna, Zebrafish) were chosen as the research object, and they were exposed to CBZ to determine its acute(96h) and chronic(21d) effects on biochemical and physiological responses. The related biochemical parameters were analyzed like antioxidant responses, morphological indices and DNA damage. The toxic effects of CBZ on Chlorella in water at 0, 0.1, 1, 10, 50, 100mg/L were studied, the toxic effects of CBZ on Daphnia magna in water at 0, 0.001, 0.5, 5, 10, 20mg/L were studied, the toxic effects of CBZ on Zebrafish in water at 0, 0.01, 0.1, 1mg/L were studied.The main results of the experiments were as follows,1. The results of CBZ on Chlorella. The 96 h EC50 of CBZ on Chlorella was determined to be 111.86mg/L. Results showed that the Chlorella was inhibited after exposure to CBZ and the morphology of the cells were elongated and deformed, the inhibition exhibited higher with CBZ enhancement. The chlorophyll content of Chlorella was also affected by CBZ, with the increasing concentration of CBZ, the inhibition effect enhanced gradually and liquid color is more shallow. The activities of CAT and SOD were activated in low concentration but inhibited in high concentration and the activities of MDA decreased with the CBZ.2. The results of CBZ on Daphnia magna. The 48 h LC50 of CBZ on Daphnia magna was determined to be 63.934mg/L. The results of SOD and GST showed that the embryo numbers, total number of eggs, body length and the intrinsic growth rate of daphnia magna were decreased with the increase of the concentration of CBZ.3. The results of CBZ on Zebrafish. The 96 h LC50 of CBZ on Zebrafish was determined to be 7.845mg/L. CBZ belongs to medium toxic substance to juvenile fish and the Zebrafish appeared a series of symptoms of poisoning such as sedimentation and unresponsive. But acute exposure results showed that the 96 h exposure of CBZ has no obvious effect on adult Zebrafish. The activities of SOD and CAT were inhibited fot the first time and the activity of MDA was activated in low concentration but inhibited in high concentration. The Olive tail Moment and tail DNA content showed that both two insecticides can cause the dose-response relationship and the DNA damage showed an increasing trend with time.These results showed that the toxicities of CBZ in Chlorella, Daphnia magna and Zebrafish were confirmed. This study provides a scientific basis for the emission threshold of CBZ in water and the toxic effect of CBZ in aquatic environment.