Synthesis Of Organic/Inorganic Carbon Materials And Their Application In Photo/Electric-triggered Biosensors

Early detection of tumor markers is crucial for the diagnosis and treatment of cancer, which also provide a good opportinity for pathological study, thus scientists all over the world regard the conquer of cancer as an important task. Biologists, medicial scientists and technical workers from all countries pay attention to reform and innovation of a variety of method, in order to achieve the goal of early, accurate and rapid detection of cancer. However, in terms of the currentinternational development situation, many remote areas in developing countries have difficulties in cancer, such as lacking in money andtechnical personnels who can operate expeisive and large-scale instruments. Therefore, to construct portable biosensors which are simple, low cost, small volume but high sensitivity, good accuracy become not only work direction of sciencitists and technology workers but also our research interest.By comparison to other analytical methods, electroluminescence（ECL） has many advantages, such as high sensivity, good reproducibility, continuous measurable, simple operation, easy-to-control, especially it exhibit unique features in biochemical analysis, pharmaceutical analysis and immunoassay. ECL sensors make their selectivity and practicability improved by immobilizing ECL reagents on the electode with further modification. Carbon materials attract a wide spread attention owing to divisity, good properties and applications in the field of analytical chemistry.In this paper, we take advantages of carbon materials in the construction of emerging ECL sensors, in order to design biosensors with good selectivity, wide linear range, and good analytical performance, to achieve the objective of the analysis, which can meet the requirements of analysis. The main research is as follows:（1） In situ growth CdTe quantum dots（QDs） on the surface of graphene with larger specific surface area to prepare QDs-graphene composites（G-CdTe）. On the basis of the characteristics of Zn Fe₂O₄@Au to catalyze reduction of H2O2 to quench ECL signal of G-CdTe. In this way, ECL analysis method was adopted on the sensitive and selective detection of carcinoembryonic antigen（CEA）.（2） Carbon nanotubes（CNTs） was acidized by concentrated acid mixture and modified with gold and palladium alloy（Au/Pd） to improve its property. Through calcining guanidine hydrochloride to synthesize bulk graphite-like carbon ntride（g-C₃N₄） and ultrasonic liquid exfoliation of g-C₃N₄ to generate g-C₃N₄ nanosheets（NSs）,which then recombined with Au NPs to form Au-g-C₃N₄ NHs. Au-g-C₃N₄ NHs possessed strong and stable cathodic ECL activity, thus was used as ECL agent to construct high performance ECL DNA aptasor to achieve high sensitive and selective detection of target DNA.（3） On ITO glass electrode substrate, synthesized polyaniline-polypyrrole（PANI-PPy） film by cyclic voltammograms scanning and eletroplated dendritic silver by constant potentiometric stripping analysis method. PANI/PPy-Ag film had good conductivity and biocompatibility and used as electrode materials. Based on the signal amplification strategy of ZnO@AgNCs, we constructed a CEA sensor to realize the sensitive and selective detection of CEA.