| Butanol, is not only an important organic chemical raw materials, but also a new biofuel with great potential. However, using the conventional acetone-butanol-ethanol (ABE) fermentation to produce bio-butanol has some questions. Due to the presence of toxic of butanol inhibition, it will result in final low product concentration and low productivity. In addition, the high cost of raw materials is another factor in the development of bio-butanol.Then an online off-gas analysis for anaerobic fermentation can be desinged. Using the online off-gas analysis, the fermentation performance of C.acetobutylicum were investigated. The online off-gas analysis system can provide detailed gas datas to analysis anaerobic metabolic process.Adding neutral red and using immobilization technology can produce a high concentration and high productivity butanol. Seaweed, is a raw materials. A method of pretreatment and enzymatic saccharification technology has been established. Then we can explore the content of reducing sugars and mannitol in seaweed, to conduct a feasible study of its energy.The design of anaerobic fermentation off-gas online system is feasible. The volume of oxygen in this system is only 5.9 mL, the volume of oxygen in aerobic fermentation off-gas online system is 123.7 mL. The aerobic fermentation off-gas online system monitoring total gas volume was 21.0 L, H2 was 7.4 L, CO2 was 12.5 L. The anaerobic fermentation off-gas online system monitoring total gas volume was 34.9 L, Hbwas 13.9 L, CO2 was 19.3 L. The total gas volume, CO2 volume and H2 volume increased by 66.2%,87.8% and 54.4% in anaerobic fermentation off-gas online system respectively.By adding neutral red electron carrier and using cell immobilization technology, we can obtain a high concentration of butanol. The study investigated the influence of electronic carrier for metabolic flow of ABE fermentation. After adding of neutral red, butanol and ABE yield were increased to 15.1 g/L and 22.2 g/L, butanol yield improved by 9.5% compared with the control in 0 h neutral red added, butanol production increased 19.0% compared with the control in 10 h neutral red added. The ratio of acetone-butanol than the control improved by 0.36. Compared to free cell fermentation, the period of FBB fermentation is shortened, butanol production significantly increased strength. Butanol highest yield was 15.4 g/L, production strength 0.64 g/(L·h-1), respectively, compared with the control group improved 12.4% and 106%, CO2and H2, respectively, the highest gas production rate increased by 60% and 9%, gas production increased by 20.7% and 41.3%.Using the method of acid hydrolysis and enzymatic hydrolysis for seaweed,we can establish the optimum conditions for seaweed production mannitol and reducing sugars. Optimal conditions for acid hydrolysis substrate concentration of 8%, acid hydrolysis time was 90 min, acid hydrolysis temperature was 120℃, acid concentration of 0.5 M. Using the hydrolysate of seaweed for the substrate, the concentration of butanol is 1.2 g/L, the butanol yield is 0.05 g/g. |
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