Study On The Improvement And Optimization Of Fermentation Process Of Citric Acid Fermentation Tank Mixing

This paper researches on citric acid fermentation process, through the test purpose is to further optimize the fermentation process control, to achieve fine control.(1) Through the testing on the existing basis of existing production strain is optimized:to determine the initial sugar concentration was 15%, the initial fermentation for 4.5 pH, amount of spore inoculation of 2.5*105/ml. Culture medium formula:Determine the liquefaction of add 16% corn liquefaction method, The total nitrogen and protein content of 0.645,0.1% urea. The optimum ventilation control before 24h, ventilation ratio is 1:0.9. the fermentation period ventilation ratio to 1:0.5.(2) Through the transformation of the mixing system to determine the six parabolic disc turbine 500 L fermentation tank is selected to cavitation inhibited leaf effective, suitable for the fermentation tank device. At the same time that the reduced diameter tube air ring, to ensure that the disk can effectively block the exhaled air, and the pore size increased and the increase in supply, is conducive to the smooth; The process can effectively improve the conversion rate was increased from 90.3% to 98.3%. residual sugar decreased from 1.05% to 0.55%, the acid production increased from 13.55% to 14.75%, cycle control from the original 64h reduced to 63h in. (3) by controlling the process fine on glucose fermentation of citric acid, effectively increase the dissolved oxygen in the fermentation process of cell metabolism, to meet the demand, and that, on the fermentation process in the production of air supply volume segmentation implementation is effective. Through the optimization of fermentation process control, the sugar acid production was 16.25%, the conversion rate was 98.48%.