The Preparation And Biological Properties Polypyrrole/Potassium Sodium Niobate Electroactive Composites

Fractures,bone defects in orthopedics is a common disease,the effective treatment is often dependent on the use of bone tissue replacement material.According to the electroactive of bone tissue,it has potential applications to promote bone tissue repair,regeneration and shorten the treatment period using electroactive biomaterials as bone substitute.In this paper,we carried out research on piezoelertric potassium sodium niobate and conductive polymer polypyrrole composites based on the theoretical basis of the electrical activity of bone tissue,cells and biological tissue.During the preparation of the potassium sodium niobate/polypyrrole composite microspheres,we explored the morphology,phase composition,effect of thermal stability,conductivity and size of microspheres by changing the pyrrole compound with potassium sodium niobate different proportions.We tested the toxicity of biological material by cell life and death stain and cell proliferation assay.Experimental results showed that when pyrrole and potassium sodium niobate ratio is 10/3,the polypyrrole/potassium sodium niobate composite microspheres having a minimum size,and the composite has good biological activity,showing the potential biomaterial applications.Considering the electrical properties of bone tissue,we have designed a conductive and piezoelectric composites.In the process of preparing a composite material,we use the template-free electrochemical method to synthetic conductive polypyrrole on the surface of potassium sodium niobate ceramic and the surface potential of composites has been measured by tomic microscope directly.The results show that the surface charge has a significant different with different poled state.In cell experiments,the material exhibits good biocompatibility as compared to the rest of the two,and promote cell proliferation with a high surface potential.In addition,we changed the morphology of polypyrrole/potassium sodium niobate composites with nano-cone、random nanonet and flat film and research the surface potential of them.The results show that the morphology of composite material does not affect its biological activity and nanonet composite has exhibited good proliferation ability.In this paper,we use the advanced scanning Kelvin probe microscopy directly characterized the surface potential of composite materials,investigating the effect of piezoelectric ceramics on the surface potential and the cell behavior on composites with different surface potential.In addition,we explored the influence biological properties with different morphology.In this paper,the results of the conductive electro-active piezoelectric composite has important reference value to the research and clinical application of bone repair materials.