Quality Effect On The Se-enriched Buckwheat Based On Results Of Several Components Analysis

Selenium acts as an essential trace element for human beings,and shortage of it will cause many diseases.As an important part in food chain,Selenium of human body comes mainly from plants.Because of grown in high altitudes area,Liangshan buckwheat contains rich protein,trace elements and secondary metabolites,with good healthcare function.But Liangshan state located in the Low-Se regions in china,Se content in buckwheat at a low level.Se-fertilization can enrich Se content in buckwheat.The absorption of selenium and effects selenium on plants are important research contents of in Selenium supplementation.This research studied the main occurring state of different parts of buckwheat absorb different valence inorganic selenium compounds and influence of Se-enriched on the contents of trace element,protein and secondary metabolites in buckwheat,evaluating quality of buckwheat.Using platen heating digestion,hydride generation atomic fluorescence spectrometry determinated selenium content in all parts of buckwheat.The results show that all parts in the control group were lower than the qualitative method detection limit;dose group of buckwheat in all parts of the plants selenium content increased significantly.The content of selenium in the dose group buckwheat:Leaf>Stem>Seed>Hull;buckwheat selenium content and spraying the amount of Se-enriched reagents had significant positive correlation.The absorptive capacity of selenate was better than selenite in buckwheat.The contents of 14 elements（Li,Cd,As,Co,Ni,Mo,Pb,V,Ba,Sr,Cu,Mn,Zn,Ti）in seed、stem and leaf of selenium enriched buckwheat and control group buckwheat were determined by ICP-MS,high pressure closed-vessel digestion.The experimental results show that the trace elements contents of buckwheat leaves and stems are greater than the content of the seeds;Se-enriched not noly could increase the contents of Zn,Mn and Mo inbuckwheat seed,but also could reduce the contents of heavy metals such as Cu,Cd,Pb and As in buckwheat.In the control group and dose group of buckwheat Cu,Cd,Pb and As are in line with the content of the medicinal plants and preparations import and export green trade standards of the content regulations.In order to study the influence of Se-enriched to protein and secondary metabolites,Kjeldahl method for the determination of the control and dose groups buckwheat seed protein content.The results showed that dose group of buckwheat seed protein content increased significantly,the highest added 30.71%.Protein content and spraying the amount of Se-enriched reagents had significant positive correlation.Apply Prussian blue and AlCl₃ spectrophotometric method to determine the concentration of total phenolic acids and total flavonids in buckwheat seed.The results showed that dose group of buckwheat seed total phenolic acids and total flavonids content increased significantly,the highest added 32.70%and 38.46%,total phenolic acids,total flavonids content and spraying the amount of Se-enriched reagents had significant positive correlation.In experiments,HPLC was used to determine the content of Rutin in Se-enriched buckwheat seed and analyze the finger-print spectras.The results showed that dose group of buckwheat seed Rutin content increased significantly,Se-enriched could increase contene of Rutin,the highest added 36.41%.Rutin content and spraying the amount of Se-enriched reagents had significant positive correlation;Compared the similarity evaluation of the finger-print spectras,when increasing Se content,the similarity evaluation of the finger-print spectras became lower;The total peak area and the common peak total area in the dose group bigger than The total peak area and the common peak total area in the control group,the highest added 16.21%and28.08%.The types and contents of secondary metabolites had significant different between the dose group buckwheat and the control group.All results above showed that,Selenium supplementation produced beneficial effects to contents of secondary metabolites in buckwheat,improved the quality and the healthcare function of buckwheat.