Construction And Performance Evaluation Of A New Oil Production Engineering Strain

With the rapid development of China's economy,the demand for oil is increasing day by day.MEOR has the advantages of low cost and no pollution to the environment,attracted widespread attention,but the scholars mainly focus on the research of single strain production effect or several strains composite production effect at present,and the gene engineering methods used in MEOR is rarely reported,so it is necessary to conduct in-depth research in this area.In this paper,we first extracted four kinds of rhamnolipid expressing plasmids from E.coli,Then,the rhamnolipid expression plasmid was transferred into the biopolymer producing strain by electroporation,three kinds of transformation strains that have the ability of producing biosurfactant and biopolymer were constructed.Then,through evaluating the productions of the transformation strains.A strain with the best performance was selected as the new oil recovery engineering strain and named WS2.Then the adaptive of this strain was evaluated,the experimental results show that the temperature range of this strain is 35~75?,the pH range of this strain was 5~10,the stability time of this strain is less than 40 d,this strain also has good salinity adaptability and good shear resistance.Finally,the oil displacement effect of strain WS2 was evaluated,the experimental results show that the oil displacement effect of the new strain WS2 is better than that of the biopolymer-producer strain SP1 and biosurfactant-producer strain BS1,and the recovery rate after water flooding could be increased by 16.66%,it is higher than strain SP1 and strain BS1 by 3.32% and 6.43% respectively.The research results provide theoretical basis and technical support for the promotion and application of microbial oil recovery technology.