The Design,synthesis And Application Of Several Novel Reaction-based Fluorescent Probes

Fluorescence detection method has been widely used in medicinal,biological,environmental detection for its relative simplicity,high sensitivity,and capable of real-time and dynamic detection in vivo.Herein,we reported the design and application of four novel fluorescent chemodosimeters for HCl O,H₂S,Hg²⁺ and HCHO,respectively:（1）A highly sensitive fluorescent probe PHC1 has been developed for the detection of HCl O.Based on the HCl O-promoted intramolecular oxidative cyclization reaction,the probe exhibits high sensitivity,good selectivity,and low detection limit for HCl O.（2）A new phthalimide-based "Turn-On" fluorescent probe PHS1 has been developed for the detection of H₂S.The probe possesses good selectivity,high sensitivity and low detection limit for H₂S.Furthermore,the probe could be successfully monitoring of hydrogen sulfide in living cells.（3）A novel coumarin-based ratiometric fluorescent probe PHM1 has been developed for the detection of Hg²⁺ in aqueous solution.The probe possesses good selectivity,high sensitivity and low detection limit for Hg²⁺.Furthermore,the probe could be successfully monitoring of Hg²⁺ in living cells.（4）A new naphthamide-based "turn-On" fluorescent probe PHF1 has been developed for the detection of HCHO.The probe possesses excellent water solubility,good selectivity,high sensitivity and fast response time（within 5 minutes）for HCHO.