Synthesis And Bioimaging Application Of Tetraphenylethene

Biological imaging(bioimaging)has become a powerful tool in biological research today because it offers a unique approach to visualize the morphological details of cells.Fluorescence imaging is one of the most powerful bioimaging techniques for real-time,non-invasive monitoring of biomolecules of interest in their native environments with high spatial and temporal resolution,and is instrumental for revealing fundamental insights into the production,localization,trafficking,and biological roles of biomolecules in complex living systems.The development of fluorescent probes has facilitated the recent significant advances in cell biology and medical diagnostic imaging.Over the past few decades,a variety of fluorescent probes,such as semiconductor quantum dots,fluorescent carbon dots,Ln ion doped nanomaterials,photo-luminescent silicon nanoparticles,metallic nanoclusters,organic small molecules and organic fluorescent nanoparticles have been synthesized and extensively investigated for biological applications.Aggregation Induce Emision(AIE)materials have been extensively studied in the field of biomimetic imaging due to their excellent optical properties.In this paper,based on tetraphenyl ethylene(TPE),a series of fluorescent materials with aggregation induced luminescent properties were synthesized and biologically imaged using the nanoparticles prepared by these materials.The specific research contents are as follows:1.Organic fluorescent probes play an important role in modern biomedical research,such as biological sensing and imaging.However,the development of organic fluorophores with efficient aggregate state emissions expanded to the red to near-infrared region is still challenging.Here,we present a series of highly efficient Far Red/Near-Infrared(FR/NIR) fluorescent compounds with aggregation-induced emission(AIE)properties by attaching electron donor and accepter to tetraphenylethene(TPE)moieties through a simple synthesis method.These compounds exhibit the pronounced fluorescence enhancement in aggregate state,the red to near infrared emission,and facile fabrication into uniform compouds-loaded Pluronic F127 NPs.The emission maximum of the NPs fabricated by the self assembly method is in the range of 550nm-850 nm and the highest fluorescent quantum yield is 15.2%.The biological imaging of NPs of compound 1 and 2 for A549 lung cancer cell indicates that these compounds are effective fluorescent probes for cancer cell with high specificity,high photostability and good fluorescence contrast.2.Based on tetraphenylethylene,a novel organic fluorescent dye TPE-2NH2 was synthesized.This material is green emision,it can be reacted and the product generated red emision the presence of NO,so this material has the nature of the detection of NO.Due to the hydrophobicity of the material,we loaded the dye into the silica mesoporous nanoparticles.The prepared nanoparticles were homogeneous and stable,with an average particle size of 120 nm,good biocompatibility and high sensitivity.The nanoparticles were co-cultured with MCF-7 cells,and red light was found in the cytoplasm.Therefore,this probe in the cell to detect NO,showing a good application value.