Studies On Enrichment And Coexistence Of Various Energy Resources In The North Margin Of Qaidam Basin And Its Reservoir (ore)-forming System

Perchlorate（ClO₄^-）is a typical persistent contaminants,which threaten to the human health because of its widely use and due to its large number of its residues dispersing into the environment.The exposure characteristics of antibiotic in the environment was "mixed-persistent-low dose",which can inhibit the activity of microorganisms,affect the material circulation and energy flow of the ecosystem,thus posing a potential threat to the stability and diversity of ecosystems.Nowadays multiple pollutants in environment is more and more prevalent environmental problem.In this study,we constructed a correlation analysis between the antibiotic resistance genes Blaoxa-1、Sul（Ⅱ）、Erm（f）、16S rRNA gene and ClO₄^-degradation.The effects of microbes on the degradation rate of perchlorate in the presence of antibiotics were explored.The mechanism of the biodegradation of ClO₄^-was explained from the genetic level.The results of this study were as follows:（1）Perchlorate degradation process is divided into three stages,namely,stable period,acceleration period and slow period.The perchlorate degradation rate was significantly affected by the concentration of antibiotics.With the increase of antibiotic concentration in the system,the microbial degradation rate of perchlorate slowed down;（2）The quantitative analysis between high dose antibiotic addition system（0、100、500、1000、5000、10000 ug/L）and low dose（100 ug/L）repeated adding system show that,in the high-dose antibiotic addition system,the resistance gene expression are positively correlated in Lincomycin in the Erythromycin system,while the Sulfadiazine system are opposite.The expression of low-dose repeated adding system and high-dose system resistance gene is different,and the microbial response to different kinds of antibiotics and different ways is different;（3）The results showed that the main dominant phylum is Proteobacteria in each system,and the representative ClO₄^-degrading genera Dechloromonas and Azospira were detected.The microbial community structure of the system was not much different from that of the original activated sludge in the high dose of antibiotics,and the diversity of the community was changed greatly in the low dose of antibiotic system,and the community structure under different antibiotics obvious different.