Preparation And Properties Study Of Novel Fluorescent Copper Nanoclusters

In recent years,with ultra small size,fluorescent copper nanoclusters(CuNCs)consisting of several or dozens of metal atoms have grown into a novel fluorescence nanomaterial.Because of their good biocompatibility,excellent photostability,high specific surface area,simple surface modification and fluorescence tuning,low biological toxicity and other advantages,copper nanoclusters have become a hot spot of research and application in the fields of biological analysis and environment detection,etc.Due to the lower chemical potential of copper,it is easily to be oxidized in the air.Preparation and properties studies of copper nanoclusters with excellent performance are still at an early stage.On the basis of related literature reviews,novel fluorescent copper nanoclusters were prepared employing amino acides and thiols as templates and stabilizers.Furthermore,we designed new fluorescence sensors with good selectivity and high sensitivity based on the prepared copper nanoclusters.The contents are as follows:1.Fluorescent and water-solubility copper nanoclusters were prepared employing L-Methionine(Met)as the template.The optimum synthesis conditions and properties of as-prepared nanoclusters such as excitation dependence,stability,pH response and temperature tolerance were studied in detail.The experimental results show that the obtained copper nanoclusters have strong fluorescence intensity,good stability and excellent anti-interference ability,which ensure their broad application prospects in sensing,cell fluorescence imaging,catalysis and other fields.2.A microwave-assisted synthesis method for preparation of copper nanoclusters(Met-CuNCs)emitting blue fluorescence was proposed.The optimal experimental conditions including concentrations and volumes of L-methionine,copper sulfate and hydrogen peroxide and the microwave parameters are investigated in detail.With the addition of dopamine(DA),the fluorescence intensity was enhanced in proportion with the increasing concentration of dopamine.According to this special phenomenon,a new signal on sensor was developed to detect dopamine.The experiment results show that the sensor has good selectivity and high sensitivity(LOD=27nM).Meanwhile,the detection of dopamine content in the actual injection sample is realized satisfactorily.3.1,4-dithiothreitol(DTT)contains two sulfhydryl groups which could be easily combined with metal ions.we proposed a simple,rapid and eco-friendly method to prepare water-soluble and stable fluorescent copper nanoclusters by using DTT as both capping agent and reducing agent with cupric nitrate as metal precursor.Meanwhile,a novel and highly sensitive sensor was developed for the determination of Co2+,according to the phenomenon that the fluorescence intensity was obviously reduced with the addition of Co2+ to the prepared DTT-CuNCs probe.The sensor could be used to detect Co2+ concentration in the linear range(0.2-3μM)with high sensitivity and selectivity.