| Curcumin as a natural extracted from traditional Chinese medicine turmeric plant polyphenols,has been confirmed with the effect of reversing multi-drug resistance of tumor,combination with adriamycin can improve the sensitivity of adriamycin on tumor cell,thereby reversing multi-drug resistance of tumor.According to the folate receptor expressed in normal tissue low and in some tumor site excessive expression characteristics,to select good biocompatibility,natural non-toxic,cheap,and biological macromolecules with a range of antitumor activity of chitosan as drug carrier,doxorubicin and curcumin as antitumor drugs,using a combination of folate receptor and its ligand with selectivity and specificity,folic acid coupling to chitosan and then combined with anti-tumor drugs,via folate receptor mediated effect,increase the concentration of the drug in lesion local,improve curative effect,reduce side effects,to reverse multi-drug resistance of tumor and the purpose of targeted drug delivery.Blank chitosan nanoparticles prepared by ion condensation method,through infrared,grain size analysis and observation on the characterization,the particle size of about 200 nm,form neat,can stability of nanoparticles in solution;Discussed the influence of different reaction conditions on the generated nanoparticles,it is concluded that the optimal reaction conditions were as follows:the concentration of chitosan and TPP were 2.5 mg/mL and 1 mg/mL,reaction temperature 25 ℃,stirring speed 500 r/min,reaction system pH value of 5.0.Established adriamycin and turmeric ferrite HPLC analysis method,used for drug-loading nanoparticles coating rate and drug loadings calculation.Was prepared by chitosan curcumin nanoparticles and double drug nanoparticles,infrared spectrum(IR)the results showed that chitosan was successfully achieved in the experiments of curcumin nanoparticles and chitosan load double nanoparticles,nanoparticles assumes the circular pattern neat,particle size of around 260 nm,chitosan carrier double drug nanoparticles china-arab drug envelopment rate and drug loadings were 87.5%and 40.5%respectively,the envelopment rate of curcumin and drug loadings were 93.98%and 43.3%respectively.With chitosan as the carrier,folic acid for the targeted receptor,sodium tripolyphosphate as polyanion,curcumin and adriamycin as drug model,using the ion electrostatic interaction between Yin and Yang,folic acid was synthesized by coupling of chitosan nanoparticles and folic acid coupling double drug nanoparticles.Measured folic acid coupling chitosan carrier double nanoparticles size 350 nm,zeta potential of + 34.76 mV,nanoparticles morphology neat and uniform size.Tested the folic acid coupling chitosan nanoparticles,the content of folic acid in when activated folic acid ester with chitosan molecules on free amino ratio of 1:1,each link the amount of folic acid on chitosan roughly 38.According to reports in the literature,in the folic acid-protein coupling on average a protein coupling three folic acid molecules have strong targeting,shows that the nanoparticles have stronger tumor targeting function.With particle size of the nanoparticles,adriamycin packet encapsulation efficiency and drug loadings,the envelopment rate and drug loadings of curcumin as an index,the response surface method is investigated,chitosan concentration,concentration of TPP,doxorubicin concentration and the concentration of curcumin on the various factors that influence the response value of chitosan concentration and concentration of TPP in certain range can form good nanoparticles,the concentration is too low or too high adverse reaction,doxorubicin concentration is too low or too high will lead to adriamycin drug-polymer interactions with curcumin drug loadings are falling,the concentration of curcumin and curcumin coating rate is directly proportional to the drug,with adriamycin is inversely proportional to the envelopment rate and drug loadings. |
PDF Full Text Request