| With the rapid development of economy,the scarcity of land resource is less and less.How to repair and use the soil with heavy metal or the salinization pollution safely has important significance.As an eco-friendly problem soil remediation technology,phytoremediation is a research hotspot at home and abroad.In this study,we took the economic crops,Oenothera glazioviana with low heavy metal accumulation in grain as research materials,for the typical five kinds of metal elements in contaminated soils including copper,lead,zinc,cadmium and sodium,to comprehensively study the patience and the potential for problems of soil remediation by the seed germination,seedling growth and the effects of Oenothera glazioviana combined with the soil microorganism factor,arbuscular mycorrhizal fungi and the chemical factor,EDDS((S,S)-ethylenediaminedisuccinic acid).Taking Triticum aestivum and Brassica campestris as references,based on seed germination experiment,according to the inhibition degree of root length,Triticum aestivum was the most tolerance to the five metals than Brassica campestris,while Oenothera glazioviana has the lowest patience of the five metals.Comparison of different metal tolerance,the root of Oenothera glazioviana could not growth normally at 25 mg L-1 Cu,25 mg L-1 Cd,0.2%Na and 125 mg L-1 Zn treatment;By 125 mg L-1 Pb treatment,the root length was significantly be inhibited and compared with the control,the inhibition rate was 85%.Pot experiment were performed in the different concentration of Cu,Pb,Zn,Cd and Na in soil by adding exogenous metal chlorides.The results showed that when adding 125 mg kg-1 Cu to soil,the copper content in shoot was up to 4.86 and 2110 mg kg-1 DW.When adding 1000 mg kg-1 Pb to soil,the shoot and root content had reached 4.88 and 2030 mg kg-1 DW.When 500 mg kg-1 Zn was added to the soil,the zinc content in shoot and root were 173 and 1230 mg kg-1 DW.When 50 mg kg-1Cd was added to the soil,the content of Cd in shoot was up to 2.62 mg kg-1 DW,while 168 mg kg-1 DW in root.When 0.5%Na adding to the soil,the sodium content of the shoot was up to 1210 mg kg-1 DW,while 3030 mg kg-1 DW in root.As a whole,the highest tolerance concentration of Oenothera glazioviana for five kinds of metal elements were 853 mg kg-1 Cu,1143 mg kg-1 Pb,1729 mg kg-1 Zn,502 mg kg-1 Cd and 0.5%Na.Oenothera glazioviana was suitable for mild level stress of Na,middle level pollution of Cu and Zn,while high level pollution of Pb and Cd.By inoculation experiment,copper solution gradient treatment experiment and root bag experiment,we explored the effects of Glomus mosseae and Glomus coronatum on Oenothera glazioviana absorption and distrbution of Cu.The results showed that:(1)Compared with the control,inoculating G.c and G.m could improve the accumulation of the biomass,but there were no significant differences between G.c and G.m.(2)Under light concentration of copper treatment,inoculating G.c and G.m could improve the Cu tolerance of Oenothera glazioviana.Compared with Gm,the tolerance of G.c to Cu was more obvious.Under the treatment of 1000 mg L-1 Cu,compared with the control,by inoculation Gc,the biomass,Cu concentration in shoot and Cu concentration in root of Oenothera glazioviana were increased by 23.1%,2.6 times and 5.0 times,while total accumulation of Cu increased by 4.2 times.(3)EDDS was beneficial to the mycorrhizal system of Oenothera glazioviana-arbuscular mycorrhizal fungi to absorb and transport Cu.Compared with the treatment group without EDDS,in EDDS treatment group(96 h),the Cu content in shoot of inoculation AMF and uninoculation in Oenothera glazioviana were increased by 80%and 2.2 times,while total accumulation of Cu were increased by 1.0 and 1.8 times. |
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