Studies On Screening Deodorant Microorganisms And Its Application In The Odor Treatment

During the processes of manufacturing,there will be a variety of odor gas and waste water that emits a large amount of stench with strong stimulation.The main components of the odor are hydrogen sulfide and ammonia.Apart from this,hydrogen sulfide and ammonia would to the detriment of people's health including people's nervous system,endocrine system,digestive system,circulatory system and respiratory system.Meanwhile,they would pollute the environment and corrode the equipment.Therefore,how to remove NH3 and H2S from waste gas has become a universe concern of the community.Compared with the conventional physical and chemical deodorization methods with high cost and serious secondary pollution,biotechnology offers the most economical and environmental method for air pollution control when dealing with the removal of odorous and toxic contaminants from.The strains with high-efficiency deodorization is the core of biological deodorization.In this study,one yeast strains and two bacteria with the good reduction property of NH3 and H2S were isolated and screened to provide efficient strain sources for the promotion and application of biological deodorization.we used selective medium to filter four yeast strains from homemade wine.After enrichment culture and dilution coated plate method,16 strains are isolated from ammonia-containing PDA medium,16 strains grown on the ammonia-containing enriched medium and 18 strains which are capable of growing on the sulfur purification medium.In order to calculate the removal rate of ammonia,we used Nessler's reagent colorimetric to determinate the ammonia content of the culture medium to screen out of high efficient strains of deodorization function.A2 can reach up to 94%removal rate and FN2,FN3,FN16 have low deodorizing efficiency with 40-60%removal rate in 36 hours.For the 16 strains isolated from ammonia-containing enriched medium,the removal rate of BN2,BN3,BN5,BN6,BN7,BN8,BN10,BN11,BN12,BN14 can reach up to 80-90%in 48 hours.BN3 can adapt to the environment easily and has high ammonia removal efficiency,since it has been able to reach 36%removal rate in 6 hours,and 8 7%.8 in 48 hours.To calculate removal rate of sulfide,we used methylene blue colorimetric assay the sulphide content in culture medium.BS11,BS14,BS15,BS17 and BS18 have high sulfide removal rates of about 90%in 9 hours,especially BS15 can reach up to the removal rate of 92%.According to determination of the physiological and biochemical properties on BN3 and BS15,and the genome sequence determination of the 16s rRNA,BN3 has been proved to be Pseudomonas sp.and has the largest similarity of the Pseudomonas plecoglossicida(100.00%).BS15 was proved to belong to Microbacterium sp.and has the largest similarity to Microbacterium binotii(99.56%).According to the determination of the optimal growth conditions,BN3 grows fast in the temperature of 30-40 ?,pH of 5.0-8.0,substrate concrntrate of 0.5-3 g/L and inoculumconcentration of 5-10%.The suitable culture condition of BS15 is in the temperature of 30?,pH of 6.0-7.0,inoculumconcentration of 10-15%and the substrate concrntrate of 1.0-2.0 g/L.Through the simulated experiment of removing H2S from BS13 by reactor,with weathered sand as filter,the height of 500 mm,the admission velocity of 4 L/min,the residence time of 40s,the concentration of H2S at the air inlet is 672 mg/m3,and the concentration of H2S at the air outlet is 2.77 mg/m3,the removal rate is over 99%.