Preparation And Application Of Fluorescent Chemical Sensor Based On Temperature Responsive Double Hydrophilic Block

In recent years,the concept of self-assembly has been introduced into the field of fluorescent chemical sensor.Better coordination and application are realized through the use of intelligent polymer as the response matrix when prepare fluorescent chemical sensor.Fluorescent chemical sensors based on double hydrophilic block copolymer with good water solubility,temperature response,metal ions or pH response,biocompatibility,and high sensitivity were designed and prepared in this subject.(1)A new kind of metal ions responsive fluorescent chemical sensor based on double hydrophilic block copolymer,PEG113-b-P(NIPAM-co-SHMa)65,was prepared.The SHMa was labeled as the metal ion recognition unit in the temperature sensitive chain.PEG113-b-P(NIPAM-co-SHMa)65 can be used as a multifunctional fluorescent chemical sensor response to metal ions and temperature.Al3+ and Zn2+ can be selectively recognised from many metal ions according to the fluorescence color and the difference of fluorescence response time.PEG113-b-P(NIPAM-co-SHMa)65 also has strong anti-interference ability,excellent water solubility,high sensitivity and excellent biological compatibility.Once the temperature rises beyond the LCST,the temperature sensitive block PNIPAM collapses,the self-assembly of DHBCs in aqueous solution will occur causing the metal ion fluorescent response element SHMa gathered inside the hydrophobic core with small polarity.Thus,the quantum yield and the detection sensitivity are both improved.In addition,the experimental results show that the polymer fluorescent chemical sensor can be used for the qualitative and quantitative detection for Al3+ or Zn2+ in biological products.(2)A new kind of pH responsive fluorescent chemical sensor based on double hydrophilic block copolymer,PEG113-b-P(NIPAM-co-SHMa-co-Rh6GEMa)62,was prepared.The basic recognition unit SHMa and acid recognition unit RhSAMa was labeled as the pH recognition unit in the temperature sensitive chain.PEG113-b-P(NIPAM-co-SHMa-co-Rh6GEMa)62 can be used as a multifunctional chemical sensor for pH and temperature with a wide range of pH detection,watersolubility,biocompatibility,high sensitivity and so on.Once the temperature rises beyond the LCST,the pH signal can be further enhanced.At the same time,the color and color difference data ΔR,ΔG,ΔB of the solution under different pH values was getted through the color recognition mobile phone APP(independent development).Two principal component linear regression quantitative model were established by SPSS 20 using ΔR,ΔG,ΔB of pH 1-6 and pH 8-14.In the end,an APP named ColorExtract was developed based on quantitative models.(3)Two new kind of metal ions responsive fluorescent chemical sensors based on double hydrophilic block copolymer,PEG113-b-P(NIPAM-co-BDMa)60,PEG113-b-P(NIPAM-co-R6GEMa)67,were papared.The BDMa and Rh6 GEMa were labeled as the metal ion recognition units in the temperature sensitive chain.The mixture of PEG113-b-P(NIPAM-co-BDMa)60 and PEG113-b-P(NIPAM-co-R6GEMa)67can be used as the multifunctional fluorescent chemical sensor response to mixed metal ions and temperature.Mixed sensors also have strong anti-interference ability,excellent water solubility,high sensitivity and excellent biological compatibility.Once the temperature rises beyond the LCST,the temperature sensitive block PNIPAM collapses,the self-assembly of DHBCs in aqueous solution will occur causing the metal ion fluorescent response element BDMa and Rh6 GEMa gathered inside the hydrophobic core with small polarity.Thus,the quantum yield and the detection sensitivity of Al3+ and Fe3+ are both improved.At the same time,the solution color and color difference data ΔR,ΔG,ΔB before and after the reaction were getted through the color recognition mobile phone APP(independent development).Four principal component linear regression quantitative models were established by SPSS 20 using ΔR,ΔG,ΔB before and after the reaction.In the end,the four models were tested and verified.