Study Of Aminated Glassy Carbon Electrode For Electrocatalytic Sensing Of Hydroxyl Compounds

With the continuous development of life science,it has put forward raised higherrequirements and challenges for analytical techniques and methods.Detecting and monitoring of the important active small molecules that related to diseases,have a more comprehensive understanding about the produce and the control of diseases.As an important electrochemical sensor,the aminated glassy carbon electrode plays a very vital role.Aminated on the electrode surface,it can effectively reduce the over potential of redox reaction and improve the electrocatalytic properties of the electrode.This way provides an effective method for selective determination.We have explored catalytic properties of the 65 kinds of hydroxyl compounds,toluene,nitrobenzene,aniline and other electroactive compounds.Based on these research,we take the selective detection of glucose for example and have discussed the performance of electrochemical and electrocatalytic of aminated glassy carbon electrode in this paper.As a basis of theory and technique for the analysis on aminated glassy carbon electrode,the study is expected to provide a new detective method in vivo detection.The main work can be summarized as follows:First,we study the electrocatalytic performance on the surface of aminated glassy carbon electrode.Glassy carbon electrode has been widely used in electrochemical methods because of its excellent electrocatalytic activity.In this thesis,65 kinds of hydroxyl containing compounds were studied to investigate the electrocatalytic effect of aminated glassy carbon electrode.There is a pair of redox peaks in the ortho and para substitution of the hydroxyl group,while the meta-substitution only have the oxidation peak,and the length of the carbon chain has no effect on the potential of the aminated electrode.The electrocatalytic properties of aminated glassy carbon electrode surface can be built according to the experimental requirements and expectations,which can lay a foundation for the future research and application of electrochemical sensing mechanism to provide ideas and methods for the design of carbon electrode in vivo applications.Second,glucose is one of the vital human physiological activity small molecules.The change of its content is directly related to human health.It has an important value to sensitive,accurate and real-time detect and monitor of glucose content in clinical medicine.It is an unremitting goal for analysts to prepare a new glucose sensor which has high selectivity,sensitivity and stability,using the appropriate method and reasonable design,.Aminated glasscarbon electrode(N-GCE)was fabricated using a one-step electrochemical technique,functionalized with glucose oxidases(GOD-N-GCE),and used as a quick and sensitive glucose biosensor.The results revealed that the novel electrode exhibited excellent electrocatalytic performance toward glucose oxidation,with a linear range from 50μM to 1.77 m M and a detection limit of 46.5μM based on a signal-to-noise ratio of 3.The GOD-N-GCE can effectively avoid the interference by other co-existence of bioactive small molecules at-0.3V,such as ascorbic acid,dopamine and so on.It is particularly important that the method can be used for the determination of glucose in real blood samples,which provides a new method and technique for the selective detection of glucose in biological samples.