The Effect Of Walnut Peptides Hypoglycemic Function

Juglans mandshurica Maxim from changbai mountain Juglans,Walnut Chinese catalpa walnut genera plants,rich in protein,fat,carbohydrates,dietary fiber and other substance has a high nutritional value.For a long time,walnuts in production has been to squeeze the walnut oil as the main direction of the production,the treatment of single nutrients and serious waste of resources,after the oil still contain rich protein hydrolysis.In the hydrolysis of protein content of up to 29.7%,titer is close to animal protein,is a kind of good protein.So with nonfat walnut pulp as raw material to the preparation of functional peptide can realize resource utilization development of plant protein sources.This paper takes degreased walnut meal as raw material,the enzymatic hydrolysis of walnut polypeptide,verified the alpha glycosidase enzyme inhibition;In human liver cancer cell insulin resistance model of glucose consumption ability strong and the weak;High sugar model of human umbilical vein in epidermal cells of protection;And establishing Ⅱ diabetes model mice fed walnut polypeptide determination of hypoglycemic effect way to get the separation and purification of hypoglycemic active peptides with good effect,specific results are as follows:(1)The inhibitory effect of peptide molecular weight determination of different concentrations of alpha glucosidase,molecular weight of 3kDa-10 kDa peptide inhibition rate was the highest,up to 67%,the molecular weight of <3 kDa is 53%,and the molecular weight > 10 kDa is the worst near to 40%;with the model of insulin resistance in human hepatocellular carcinoma cells and glucose consumption capacity determination of different molecular weight peptides of glucose consumption ability,the results showed that <3 kDa and 3kDa-10 kDa two component can improve the insulin resistance of human hepatocellular carcinoma cell model under the glucose consumption capacity;through the protection effects of different molecular weight peptides on human umbilical vein endothelial cell induced by high glucose injury that <3 kDa and 3kDa-10 kDa two components can be different the increase in NO and t-PA content and decrease ET-1,PAI-1 content and has a significant difference(P < 0.05).(2)To establish a mouse model of type II diabetes,according to the low middle and high dose(200,500 and 800mg/kg BW)mice,measured weekly water quantity and weight change,continuous intragastric administration for 30 days,fasting 8h eye blood,liver and glucose determination index.The results showed that middle and high doses of peptides could significantly reduce the blood glucose and lipid levels in model mice.By way of measuring the hypoglycemic insulin in mice model,liver glycogen content and liver glucokinase content of walnut polypeptide was speculated to improve insulin resistance,improve liver glucose utilization and metabolism,enhanced glucose metabolism,thereby reducing blood glucose to hypoglycemic effect.(3)The molecular weight by Sephadex Sephadex G-25(3kDa-10kDa)peptides were isolated and purified fractions B1,B2,B3,human hepatoma cells by glucose consumption ability is validated by good hypoglycemic component B2 were further purified on Sephadex Sephadex G-15 pure B2 component is only one peak,the peak the tip of a freeze dryer into powder storage.For the specific peptide structure of walnut peptide,further study is needed.