Bio-coal Cinders For The Treatment Of Antibiotics Wastewater

Antibiotics are the most widely used drugs in human daily life and aquaculture,and thus increase the risk of entering the environment and endanger human life and health.It can be detected in a wide range of environmental media,such as sewage treatment plants,surface water,sludge,sediment,soil and biological samples,and even in groundwater and drinking water.A bio-coal cinders immobilization process was successfully developed using solid waste coal cinders selected as the microbe support based on the screening of antibiotics degrading-microbe consortium and optimizing the operating conditions.It is expected to find a novel technology for the treatment of antibiotics by means of the synergestic cooperation of the bio-abiotic process,providing a new method for the reutilization of coal cinders.As a kind of model antibiotics,chloramphenicol(CAP)was selected as the main pollutant.The investigations in the work was concluded as follows:(1)A microbe consortium CAP degrading was successfully screened from the sludge of the polluted river in the campus of Qilu University of Technology.The optimized conditions for microbe growth and CAP degrading was investigated as the following: illumination intensity 3000 lx,temperature 30 ?,pH 7.0,DO 0-2 mg/L,inoculation amount of 20%.Under the optimum conditions,higher treatment efficiency of CAP was achieved in 7 d when the initial concentration was between 20 mg/L and 50mg/L.With the initial concentration of CAP increased,the degradation rate was inhibited,the results indicated that the microbe consortium was effective for the the degradation of CAP with low concentrations.(2)A bio-coal cinders process was developed for the degradation of CAP with low concentrations due to the preferential retention of biomass.The morphological and phylogenetic analysis was conducted by UV-vis spectra,SEM.The optimum immobilization conditions was found as: coal cinder dosage 30 g/L,the particular size between 10 and 100 mesh,and pH 7.0.It was found that the degradation efficiency of CAP in process of the bio-coal cinders was better than that of the process of sole free microbe consortium or sole coal cinders.(3)A continuous acclimatization process with 5 running cycles was applied for the increasing the treatment efficiency of CAP with high concentration of 100 mg/L.Comparing with the initial bio-coal cinders process,the treatment efficiency wasincreased form 59.3% to 98% under the optimum conditions of the coal cinder dosae 40g/L,the particle size between 20 and 100 mesh and pH 7.0.The results revealed that the acclimatization was feasible for the improvement of the degradability.Additionally,it was found that the bio-coal cinders process was available for the degradation of?-lactam antibiotics amoxicillin.(4)The bacterial diversity and community analysis of the microbe consortium was achieved by using 16 S rDNA-Illumina high-throughput sequencing technology.The results indicated that Rhodopseudomonas and Synergistetes strains dominated in the original microbe consortium,and Pseudomonas aeruginosa,Pseudomonas aeruginosa,Delphonia and Sphingomonas mainly existed after acclimatization process.(5)The surface characteristics of coal cinders was investigated by BET.It was found that the coal cinders with particular size of 10-100 mesh showed high treatment efficiency for the removal CAP even though the surface area was less than those of others.It should be attributed that the higher stability due to the immobilization system.Otherwise,the unsuitable particular sizes would do side effects on the mass transform.The results further revealed that the synergestic effects of microbe consortium and coal cinders for the higher treatment efficiency of CAP rather than the adsorption of coal cinders.The significance of this work could be concluded:(1)The bio-coal cinders has a reliable engineering application prospects due to the simple,feasible and cost effective.(2)It is characterized by the solid wastes reutilization for the treatment of CAP wastewater.