Preparation Of Micro-Nano Fibrous Film On SEBS Surface And Its Hemocompatibility

Here,we present a facile method to construct a hydrophilic,3D hierarchical architecture on the surface of SEBS[poly(styrene)-block-poly(butylene)-block-poly(styrene)]with SEBS/P-F127[poly(oxyethylene)-b-poly(oxypropylene)-b-poly(oxyethylene)]microfibers by electrospinning.The properties of the SEBS/P-F127 nano/microfibers,for example,surface morphology,wettability,protein adsorption and platelet adhesion etc,are investigated;The influence factors of relative humidity to P-F127 self-assembly behavior is analyzed during electrospinning.In addition,a nano/microfibers membrane with SEBS/A-F127/AA-2G(Vitamin C)is fabricated on the surface of SEBS via coaxial electrospinning,The surface morphology and the controlled release of AA-2G are investigated.A-F127 is synthesized by conjugating acrylamide onto the terminal hydroxyl groups of Pluronic-F127,the successful acrylamidation is confirmed by FTIR and H-NMR spectra.The strategy is based on electrospinning of SEBS/Pluronic-F 127(A-F 127)fibers onto the surface of SEBS,the influence factors of surface morphology and properties are discussed,generation of a hydrophilic surface through self-assembly of microfibers,and reduced protein adsorption and platelet adhesion.Fibers without bead formation can be observed by SEM.However,the crosslinked fibers are immersed in distilled water for 10 days and the surface of fibers becomes rough,indicating a small number of acrylamidized Pluronic-F127(A-F127)fell off the fiber.The nanofibers are electrospun at higher relative humidity(RH),because of the self-assembly of SEBS/A-F127 fiber.Core-shell structure of the fiber can be clearly observed in the SEM images.A nano/microfibers membrane with SEBS/A-F127/AA-2G(Vitamin C)is fabricated on the surface of SEBS via coaxial electrospinning,and core-shell structure is confirmed by TEM.We are prepared of SEBS/A-F127/AA-2G core-shell nanofibers and study its anti-hemolysis properties.The crosslinked electrospun meshes(3D hierarchical architecture)is constructed on the surface of SEBS with SEBS/A-F127/AA-2G by reactive electrospinning,and the release of AA-2G controlled by thermo-responsiveness of microfibers,and then the controlled release of AA-2G is measured.Thus,the blood-contacting surface reduces both mechanical and oxidative damage to RBC membranes,This work paves a new way to fabricate high blood-compatible biomaterials for RBC storage.