| Cabbage?Brassica oleracea L var capitata L?is one of the important cole crops cultivated world-wide.It is difficult to improve some important agronomic traits as its complex genetic basis.Moreover,many important agronomic traits were quantitative traits,such as bolting traits?flowing traits and yield traits.It is necessary to exploit existing genetic variation to dissect agronomic and quantitative traits in Brassica oleracea at molecular levels.In this study,using high generation of inbred lines R4P1?early bolting,and late-ripening?and R2P2?late bolting,and early-ripening?were used as the materials to construct the recombinant inbred line population?RIL8?,a high density linkage genetic map of Brassica oleracea,and analysis the QTLs related to bolting time in cabbage.Based on the self-incompatibility,DNA sequencing and blast analysis were used to quickly identify S-haplotypes of 52 cabbage accessions,and to assist in the preparation of hybrid combinations.The main results are as follows:1.Construction of genetic linkage map in CabbageSoftware JoinMap4.0 was used for genetic linkage using 408 SSR and In Del molecular markers.On the condition of the measured LOD greater than or equal to 3.0,a total of 387molecular markers were mapped into 9 linkage groups of Brassica oleracea.The number of linkage groups was the same with the number of chromosomes in cabbage,named as Ch1-Ch9.In the 9 chromosomes,the coverage of genome was 838cM with an average interval distance of 2.2 c M between markers,the length respectively was 105 cM?67 cM?103 cM?101 cM?95 cM?61 cM?94 c M?105 c M?108 cM,and the number of markers was 37?25?29?54?33?34?86?51?38.Among them,the number of C09 markers was the longest but not the longest,the C07 was the largest in number of markers,and the density is largest.2.QTL mapping and analysis of bolting traits in Brassica oleraceaThe QTL mapping and genetic effects of bolting time traits of recombined inbred lines 8?RIL8?were analyzed using the software MapQTL6.0.Field drawing time of the compositional populations was investigated,and newly constructed genetic maps were used to extract the traits.A time-related QTL?qbt-2-2?was mapped on chromosome 8,with a contribution rate of 9.1%,an additive effect of-1.23,and a molecular marker of CB10139.3.Identification and analysis of 52 self-incompatible materials for Brassica oleraceaTwo pairs of primers,SRK1F/R and SRKSP2F/R,were developed for quickly identifying the haplotypes of 52 materials.Among them,13 kinds haplotypes were found,including 2 S2 haplotypes,2 S5 haplotypes,2 S6 haplotypes,2 S7 haplotypes,2 S8 haplotypes,1 S14 haplotypes,18 S15 haplotypes,5 S28 haplotypes,6 S33 haplotypes,3 S36 haplotypes,4 S45haplotypes,3 S50 haplotypes and 2 S57 haplotypes.The frequency of different S haplotypes in this study differed from 0.19%to 34.62%.S15 was the most common haplotypes,and S14 had only one lowest material.S2,S5,S6,S7,S8,and S57 had two materials,respectively,with the frequency of 3.8%. |
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