Extraction And Separation Of Curcuminoids From Curcuma Longa L.

Nowadays, Curcuminoids become one of the hot topics in the medicine field for their good bioactivities, which are mainly extracted from Curcuma longa L.. Quantitative and qualitative determination methods were established to analysis the turmeric extract. After optimization of extraction process, the turmeric purification was studied by macroporous resin. All the studies were aimed to make a difference for developing the anti-cancer new drug of turmeric. The detail work was listed below.Firstly, the High Performance Liquid Chromatography(HPLC) method for simultaneous determination of bisdemethoxycurcumin(BDMC), demethoxycurcumin(DMC) and curcumin(CUR) was set up. It used chromatographic column of WatersAtlantic T3(4.6×250 mm, 5 microns), acetonitrile-4% acetic acid solution(47:53) as mobile phase with rate of 1 mL/min, detection wavelength of 425 nm and column temperature of 30?. HPLC-MS/MS method was also optimized to determine 19 curcuminoids from the extract. There were three kinds of isomers of curcumin identified, the structures of which were speculated as presumably chain-type, ring-type and enol-type, respectively.Secondly, microwave-assistant extraction process of wet turmeric powder was studied by single factor test, the Design of the CCD Response Surface method and Support Vector Regression model. The optimal process was obtained, using 69% ethanol solution as extraction solvent, while the specific operating condition were liquid-solid ratio(mL:g) of 21.8, microwave time of 55 s. The result turned out total extraction yield of curcuminoids is 36.38 mg/g, and the total of BDMC, DMC and CUR was 28.97 mg/g. Comparing with the optimal process of ultrasonic and reflux extractions, it was found that curcuminoids identified from 3 extract were almost the same and the contents of curcuminoids including BDMC, DMC and CUR were similar.Finally, the turmeric extract were purified by NKA-9 resin which was optimized from 8 different resins including the polar ones, weak-polar ones and nonpolar ones. By study of ethanol concentration and flow velocity of adsorption and elution, the purification process was determined, using 5 BV(ratio of liquid to adsorbent) of 60% ethanol turmeric solution with pouring velocity 2 BV/h and 1 BV of 40% ethanol solution, 1 BV of 60 % ethanol solution, 1 BV of 80% ethanol solution, 5 BV of anhydrous ethanol with velocity of 4 BV/h for gradient elution. It turned out that the total content of BDMC, DMC and CUR rose from 16.88% to 41.82% after the process, and the recovery rate was 73%. After 3 batches of verification, the purification process was confirmed to be highly stable. What's more, the purified turmeric was identified by HPLC-MS and found most curcuminoids hadn't been wiped off, while some impurities did. In addition, adsorption isotherm curve, static adsorption kinetics and the mass transfer process of dynamic adsorption were studied. The results showed NKA-9 resin adsorption isotherm was in accordance with Langmuir equation, and the dynamic adsorption was surmised as a combination of two steps. It was believed the first step was controlled by intra-particle diffusion and the left was conformed to pseudo-second order kinetic equation.