A Study Of Serum Proteins Differentially Expressed In Patients With Oral Lichen Planus Before And After Huashi Xingyu Qingre Decoction Treatment

Objective:To detect the different proteins in the serum of oral lichen planus(OLP) patients before and after Huashi Xingyu Qingre decoction(HXQD)treatment,then to investigate the treatment effect of HXQD and the interaction mechanism of HXQD and OLP.Methods:1 The serum of oral lichen planus patients before and after 8 weeks of HXQD treatment was collected,and total protein were extracted by phenol extraction.Bradford method was used to determine the protein concentration.Screened and identified the differences of serum proteins of oral lichen planus patients before and after therapy by two-dimensional fluorescence difference gel electrophoresis(2-D DIGE).2 Identified the different proteins screened by 2-D DIGE and liquid chromatography-mass spectrometry(LC-MS).3 Detected the the different expressions by the methods of Western blot and ELISA.Results: 1 There were different expressions of serum proteins in oral lichen planus patients before and after HXQD therapy,including Haptoglobin,Human Antithrombin Ⅲ,C1 complement and Vitamin D binding protein;2 After therapy the expression level of Haptoglobin was increased and Human Antithrombin Ⅲ was descended compared with before therapy by the methods of Western blot;3 The result of ELISA detected Haptoglobin was same with Western blot.The differences were statistically significant.Conclusions:1 Different proteins are well characterized in serum of oral lichen planus patients before and after HXQD therapy,including haptoglobin,Human Antithrombin Ⅲ,C1 complement and Vitamin D binding protein.2 After therapy the expression level of Haptoglobin is increased and Human Antithrombin Ⅲ is descended.The mechanism of HXQD decoction on oral lichen planus has certain connection with these proteins.