| Objective: To reveal the epidemic characteristics and the virus spectrum of respiratory viruses in hospitalized children with acute lower respiratory infection(ALRTI)in Shijiazhuang area,and provide basic data and scientific evidence for the diagnosis and the prevention of ALRTI.Methods:1 Bronchoalveolar lavage fluid(BALF)specimens were randomly collected from 558 children diagnosed with acute low respiratory tract infections,who were hospitalized in children's hospital of hebei province between March 2015 and February 2016.2 We extract the nucleic acid of all BALF specimens above.15 kinds of respiratory viruses were detected by multiple real-time fluorescence quantitative Polymerase Chain Reaction,and types and subtypes were identified by a computer software.3 All the results were statistic analyzed with Excel and SPSS17.0,using2?test and ?=0.05.Results: 1 Among 558 BALF samples,the total virus detection rate was 46.77%(261/558),and HRV was the most common virus.The detected rate of HRV was 12.01%(67/558),followed by PIV3,at a rate of 10.75%(60/558),RSV was 7.89%(44/558),ADV was 6.99%(39/558),IFVB was 3.58%(20/558),HBoV and MPV were 3.41%(19/553),IFVA was 2.51%(14/558),PIV1 was 2.32%(13/558),OC43 and 229E/NL63 were 2.15%(12/558),PIV4 was 1.97%(11/558),and PIV2 was 0.72%(4/558).No EV and MERS was detected.2 The detected rate of males and females were 47.08%(162/342)and 46.30%(100/216),respectively,there was no significant difference between male and female(2?=0.047,P =0.828)in the detected rate.And there was no significant difference in male and female of various viruses(P > 0.05).3 The detected rate was 54.55%(42/77)in children under 12 months,63.73%(65/102)at age from 1year to 2years old,56.45%(35/62)at age from 2 to 3 years old,39.06%(25/64)at age from 3 to 4 years old,41.30%(19/46)at age from 4 to 5 years old,and over the age of 5 years old,it was 36.23%(75/207).The positive detected rate in various age groups had significant differences(2?=33.374,P < 0.001),especially in the age of 1 year to 2years old children whose positive virus detected rate was the highest.And the age distribution of different viruses had differences.And distribution of the detection rate in different virus have their own characteristics in various age groups.4 Virus can be detected in each month,the positive detected rate in February 2016 was the highest,at 75.00%.There was remarkable seasonal distribution of the detected rate of total viruses in Shijiazhuang area(2?= 135.953,P < 0.001),and the peak prevalence of viruses were winter and spring,at the rate of 54.40% and 53.47% respectively and autumn was the lowest.5 62 children in 261 were found to be co-infected with multiple respiratory viruses(The positive rate of co-infected was 23.75%),including 8 triple infection and 2 quad infection.HRV,PIV3,RSV and MPV were the most common co-infected virus.The co-infection detected in each month and the months with the highest detected rate was between January 2016 and February 2016.Conclusions:1 From March 2015 to February 2016,the positive rate of virus detection of hospitalized children with ALRTL was 46.77%.2 The top five viruses that caused children ALRTI in shijiazhuang area wereHRV,PIV3,RSV,ADV and IFVB.In addition,newly discovered viruses,such as HBoV,HMPV,HCoV-229E/NL63,HCoV-OC43/HKU1,also had a high detection rate.3 The detected rate was no significant difference between male and female.The detected rate was significant difference in the age distribution,especially in the age of 1 year to 2 years old.4 There was remarkable seasonal distribution of the detected rate of total viruses in Shijiazhuang area,the peak prevalence of viruses like RSV,IFVA,IFVB,ADV,HCoV-OC43/HKU1 were in winter and spring.The peck of HCoV-299E/NL63 and MPV were in winter,HRV in spring and autumn,PIV3 in spring and summer and HBoV in summer and winter.5 Multiple real-time fluorescent quantitative PCR is a rapid,simple method in monitoring respiratory virus infection,which can reduce laboratory pollution and is worth promoting. |
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