Study The Mechanism To Improve Cognitive Function Of Rats In Alzheimer Disease Of Taking Jujubodide With Suitable Time

Objective:Taking jujubodide with suitable time in this experiment,compare the effect of cognitive function and sleep of rats in Alzheimer disease in different time and reaserch the mechanism of Alzheimer disease in order to give evidence of treating sleep and cognitive function of Alzheimer disease in taking jujubodide with suitable time in clinical.Methods: Choosing 48 elderly male SD rats,rats were randomly divided into normal group,model group,Mao Shi group and You Shi group,each group has 12.We inject D-galactoserat subcutaneously and Aβ1—42 into the hypothalamus of rats.After the success of the building model,evaluate model by using macroscopic signs observation,Morris water maze test and monitoring the number of autonomic activity of rats.At the 49 th day,Specimens are collected.Take the blood from the heart of the rats,then centrifugal serum.finally measure the concentration of MT、 MTR、5-HT;Do rats hippocampal tissue biopsy,HE staining,and observe hippocampus morphology,ultrastructure change.Do immunohistochemical detection of p-hippocampus tau protein expression,the final data analysis.Results :(1)In the navigation test of Morris test,The time of model group to collision was obvious higher than normal group(P<0.05),The time of maoshi group and youshi group to collision were lower than model group(P<0.05),The time of maoshi group to collision was obvious lower than youshi group(P<0.05).In the spatial probe test,timesthat rats went Through the hidden platform in one minutes,model group were less than normal group(P<0.05),maoshi group and youshi group were more than model group(P<0.05),maoshi group were more than youshi group(P<0.05).(2)The concentration of MT,MTR,5-HT showed model group was lower than normal(P<0.05),maoshi group and youshi group were higher than model group(P<0.05),maoshi group was higher than youshi group(P<0.05).(3): HE staining: cellular structure of normal group is clear,Big round,colored clearly,neuron cells are close and neat.Cellular structure of model group is fuzzy,nucleus are swelling and fragmentation,shape is irregular,a large number of neuron are degeneration,nuclear is pyknosis.cellular structure of Mao Shi group and You Shi are clear,the nucleus are swelling and fragmentation less.Morphological rules,the neurons are arranged more neatly,Mao Shi group is closer to the normal group.(3)Immunohistochemical: neuronal cytoplasm of model group rats in hippocampal CA1 area are tan,positive cells were significant higher than normal group(P<0.05),neuronal cytoplasm of Mao Shi group and You Shi rats in hippocampal CA1 area are light yellow,positive cells were significant lower than the model group,(P<0.05),the positive cells,Mao Shi group was obvious lower than You Shi group(P<0.05).Conclusion: 1 cognitive function of rats in Alzheimer disease of taking jujubodide with suitable time get good effect,Compared Mao Shi group and You Shi group in the cognitive function and sleep of rats,Mao Shi group is better than that of You Shi 2,With the raise of the concentration of the serotonin(5-HT),melatonin(MT),melatonin receptor(MTR),AD rats sleep is improved,so as to improve the symptoms of cognitive function in AD rats.4,Jujubodide can effectively reduce the the hippocampus Taup S199 positive cells expression rate of model group and delay the extent of excessive Tau protein phosphorylation,which in turn improves cognitive function in model group rats.