The Change And Significance Of Th17 And Treg Cells In Peripheral Blood Of Osteosarcoma Patients

BackgroundOsteosarcoma(OS)is a highly malignant primary bone tumors,its mortality and disability rates are very high,currently ranked first of all malignant bone tumors.With the development of the understanding of the occurrence and development of tumor and surgical techniques,the 5 year survival rate of OS increased from less than 20% to 50% to 60%,and more than 90% of the limb salvage treatment of patients with OS is feasible;however,more than 50% of patients may still be lung metastasis is the main cause of death in patients.For patients with pulmonary metastases and local recurrence of OS,the prognosis is very poor,there is no effective method to limit the survival rate of patients with OS to further improve the "bottleneck" problem.Exploring new therapeutic methods and searching for more effective treatment mode has been the relentless pursuit of the academic circles,and it is also an urgent need for the development of society.Today,the pathogenesis of OS is still not clear,but with the continuous development of tumor immunology,T cell immune function on the incidence of OS has been more and more attention.CD4+IL-17+Th17 derived from CD4+T cells,their functions are antagonistic to maintain the stability of the body microenvironment,and excessive expression of inflammatory factors,destruction of the Treg and Th17 cell balance,resulting in the occurrence and development of tumor.Because Th17 and Treg play a key role in the development and metastasis of tumor,the relationship between them and OS is discussed,which provides a new direction and basis for the treatment ofOS.ObjectiveThrough the detection of OS patients week blood CD4+IL-17+Th17cells and CD4+CD25+Foxp3+Treg cells and IL-17 levels and alkaline phosphatase(ALP),clear in OS patients peripheral blood Th17 and Treg cells change.Preliminary study on the changes of Th17 and Treg cells on the incidence and development of OS and to reveal the molecular mechanism of the pathogenesis of OS provides theoretical and experimental basis.MethodIn OS patients and control group as the research object,using flow cytometry(FCM)to detect the expression of peripheral blood CD4+IL-17+Th17 cells and CD4 + CD25 + Foxp3 + Treg cells rate and using the enzyme-linked immunosorbent assay(ELISA)was used to detect the serum IL-17 levels,and observe the level of ALP in OS patients admitted for routine blood tests.The data obtained by SPSS 18 statistical software for statistical analysis of data were expressed as standard deviation of measurement data(x+ s)said that the two groups were compared using two independent sample t test,with P<0.05 as the difference was statistically significant.ResultOS patients with peripheral Treg cell expression rate was significantly higher than that of control group,the expression of OS group and control group were respectively: 10.252 + 1.294% and 5.859 + 1.439%(P<0.05);OS patients with peripheral Th17 cell expression rate was significantly higher than the control group,the expression of OS and control group were respectively: 0.540% and 4.017.2.163 + 0.600%(P<0.05);Th17 cells and Treg cells in OS patients was significantly positively correlated with ALP,the R values were 0.892 and 0.805;further analysis of two groups of Th17/Treg ratio,Th17/Treg ratio in OS group compared with the control group,the high,but compared to no obvious statistical significance,OS group and control group were 0.394 + 0.047 and 0.375 + 0.077(P > 0.05).In addition,IL-17 all serum samples in lower level,only a small amount of(n=2)measure,more than ELISA in this experiment were lower than the detection threshold.ConclusionOS in the peripheral blood of patients with the number of Treg cells and Th17 cell ratio was significantly higher than the control group,OS group and two T cells were associated with ALP in the immune suppression caused by the related cue induced the occurrence and development of OS and Th17 may cause a change in the proportion of cells and Treg cells;Th17/Treg had mild changes,but the difference was not statistically significant,considering with the number of samples is less;the serum IL-17 level is very low,less than the existing ELISA kit to detect the minimum threshold,this test method is not sensitive,for higher sensitivity required for the research on the content of serum IL-17.