Comparative Study Of Pharmacodynamics And Pharmacokinetics Before And After Processing Xanthii Fructus

Xanthii Fructus,which firstly sets out in the Qianjinshizhi and belongs to Xanthium sibiricum Patr,is also known as Bring healing,sheep grass,sticky sunflower,Xanthium tree,etc.Ripe fruits with involucre are warm in nature and tastes bitter.Also,they are regarded as important medicines to treat rhinitis syndrome and arthralgia as they can be used for dispelling wind and dampness and relieving the pain.Herbal preparation said that Fructus Xanthii is warm in nature and it can dispel rheumatism from head to foot,also it can cure headache dazed,tooth pain,nasosinusitis,limb spasm pain,spasm,scrofula,sores epilepsy,partial body itching etc.Clinically,it is often used to treat allergic rhinitis and other allergic diseases,anti hypersensitivity and immunosuppressive effect of Fructus Xanthii tips.Since ancient times,herbs should be fried and removed thorns before use because it is poisonous.In the modern research,it can be found that fried Fructus Xanthii can significantly reduce virulence,while the chemical constituents and pharmacological effects of changes before and after processing has not been reported.Therefore,a comparatively study on the before and after processing of Fructus Xanthii effect and Siberian cocklebur fruit into the blood after the green original acid and cryptochlorogenic acid composition changes will be concluded.Mainly by the following:1.Study on the anti-inflammatory effect of Fructus Xanthii before andafter processingUsing xylene induced ear swelling in mice inflammation.Fructus Xanthii and fried products low dose group was 500mg·kg-1(g crude drug / kg),high dose group for value 1000mg·kg-1(g crude drug / kg),mice were gavaged for a week,the mice were killed,cut along the baseline of auricle of ear,with a hole punch punching lugs underat the same site,8mm in diameter,weighing on the analytical balance.Calculated mice ear swelling and inhibition rate.The results show that Fructus Xanthii raw product high dose group and Xanthium fried products significantly inhibited the mouse ear swelling induced by xylene in the high dose group,Fructus Xanthii crude low dose group and Xanthium fried product no low dosage group.The difference was not statistically significant,which in raw Fructus Xanthii goods anti-inflammatory effect in high dose group best.2.Study on the analgesic activity before and after the processing of Fructus XanthiiAccording to the screening results of anti inflammation experiment,analgesic experiment was carried out in mice induced by acetic acid.After administration of 0.5ml1 h in mice,Intraperitoneal injection of 0.7% acetic acid0.lm/l0 g.Injection of 15 mints in mice writhing.The Results of that the Fructus Xanthii crude high dose and high dose of Xanthium fried with obvious analgesic effect in mice.The inhibitory rate of the mice was reached 32.26%and 30.82%.The raw materials of low dose of Fructus Xanthii and the fried with low dose of Fructus Xanthii which nalgesic effect is not obvious.3.Study on hypoglycemic effect on experimental diabetic mice ear before and after processing Fructus XanthiiDiabetic mice model with four oxygen,hyperglycemia Model 72 mice were randomly divided into 6 groups,Divided into Xanthium crude high dose group(11.11g/kg),Fructus Xanthii crude low dose group(3.70g/kg),Xanthiumfried with high dose group(11.11g/kg),Xanthium fried with low dose group(3.70g/kg),Continuous administration for 28 days,By measuring the mice glucose,triglyceride,total cholesterol and other 8 indicators,the results have a certain degree of decline.The results show that the frying Xanthium has hypoglycemic effect before and after,and the effect of reducing blood sugar after processing is better than before processing.4.Fructus Xanthii chlorogenic acid and green original acid hidden pharmacokinetic studyTo take the way of filling the stomach in rats,The use of animal pharmacokinetics and other research means,The results showed that the processed products of Fructus Xanthii after intragastric administration,Neochlorogenic in 2.94 + 0.18 h peak,Half-life of 235 + 1.11 h,Siberian cocklebur fruit raw materials after intragastric administration,neochlorogenic acid in 3.75 + 0.46 to peak,the half-life of 1.70 + 0.61 h.Xanthium processed products by gavage and cryptochlorogenic acid at 3.00 + 0.46 h reached peak,the half-life of 197 + 0.66 h.Siberian cocklebur fruit raw materials by gavage,cryptochlorogenic acid in 275 + 0.27 peak and has a half-life of 2.12 + 0.68 h.results show that processed Fructus Xanthii neochlorogenic acid fast onset of action,continuing a long time;and cryptochlorogenic acid slow onset,time short.