Study On The Processing Technology Of Deep-fried Atractylodis Rhizoma And Study On Pharmacodynamics And Chemical Composition Of Atractylodes Lancea Before And After The Deep-fried

Rhizoma atractylodis is commonly used in clinical medicine,and it has a long history of processing methods.there are 18 classes of the main processing methods described in ancient materia medica and there are mainly bran fried,Chaojiao,Migan Shui system and other methods described in today materia medica.All over the country of processing specification in addition to individual regions retained soil fried Atractylodes rhizome,for prepared Rhizoma Atractylodis,bran fried Atractylodes rhizome and focal Atractylodes rhizome is the most commonly used types of pieces.However,the current research on Atractylodes mainly concentrated in fried with bran,little research on deep-fried products.Rhizoma atractylodis raw product has temperature drying and tangy,dry wet,strong wind and cold ability.After the Fried coke can alleviate rhizoma atractylodis dry sex,strengthen the spleen and anti-diarrhea effect.The effect of clinical practice has proved,but its processing mechanism is still not clear,thus limiting the further development and application of Atractylodes rhizome.Based on the above point of view,As a part of National Natural Science Fund Project “study on material basis and processing machanism of Be good at GUCANGZHIXIE of deep-fried Rhizoma Atractylodis ”(81541080).This article will focus on Atractylodes volatile and nonvolatile portion of pharmacodynamic and changes in chemical composition,to lay the foundation for the study of mechanism and material basis of Atractylodes Jianpizhixie before and after the deep-fried parts.By orthogonal experimental design method and multi-index comprehensive evaluation analysis to select the processing technology for deep-fried Atractylodis Rhizoma.Comprehensive evaluation of the experimental results,through the factor of temperature,time,stirring frequency,and the index of diarrhea index,tannin content to select the best processing technology.The best deep-fried technology was as following: the processing temperature was 220—230 ℃,turning frequency was 50 times/min,and stir-frying time was 6 min.Stir frequency has no significant difference in the three study factors.To clarify aqueous extract pharmacodynamic differences,studied the focal spleen anti-diarrhea effect and dry wet effect of Atractylodis Rhizoma and deep-fried Atractylodis Rhizoma.By diarrhea pixu in rats model to study the spleen anti-diarrhea effect of raw rhizoma atractylodis and deep-fried rhizoma atractylodis.Results indicate that under the same dose students,the gastric residual rate and TNF alpha are higher than that of coke and the small intestine advance rate,motilin,gastrin and IL-10 are lower than coke.The above results show that the spleen anti-diarrhea effect of deep-fried product is stronger than raw product.By wet resistance card in rats model to study the eliminating dampness effect of raw rhizoma atractylodis and deep-fried rhizoma atractylodis.Results indicate that under the same dose students,the deep-fried product’s content of AQP2 is higher than the raw product in the urine,its content of AQP3 in colonic mucosa is lower than raw product.The above results show that the dry wet effect of raw product is stronger than deep-fried product and the Anti-diarrhea effect is weaker than deep-fried product.The above conclusion is consistent with the clinical practice.To make clear the differences of toxicity of raw Atractylodes and deep-fried Atractylodes,the volatile oil’s LD50 of Atractylodes lancea before and after the deep-fried parts is measured by Karber’s method.The results showed that the volatile oil of raw’s LD50 is 2970mg/kg and its 95% confidence limit is 2592mg/kg~3411mg/kg.The volatile oil of deep-fried product’s LD50 is 3360mg/kg and its 95% confidence limit is 3032mg/kg~3717mg/kg.According to the compound oral acute toxicity grading criteria: the toxicity of raw and deep-fried product is low,but the toxicity of deep-fried product is lower than raw,which shows that toxicity decreased by deep-fried technology.In order to clarify the differences of chemical constituents of raw and deep-fried Rhizoma atractylodis,studying on the chemical constituents of water extracts and volatile oil of raw and deep-fried Rhizoma atractylodis by HPLC and GC respectively.The results showed that the higher content of chemical components in water extract and volatile oil was significantly different before and after processing.The above research results to further explore the correlation between efficacy and changes in chemical composition,analysis of the change of deep-fried Atractylodes in the material foundation,the final explanation processing mechanism laid the foundation.