Research On Extraction Separation And Physiological Activities Of Gynura Procumbens

Gynura Procumbens(Lour.) Meer(GPM) is an important plant with bioactive components such as triterpenoids, flavonoids. GPM has many physiological, such as anti-hyperglycemic, tumor inhibition and oxidation resistance. In 2012, GPM was approved as “a new resources of food”, but so far, it has little depth research. In the present study, the main materials were GPM with different methods of extraction, separation, purification by macroporous resin and physiological activities. The research provides foundation for development and utilization of GPM, the main findings as follows:1. Ultrasonic extraction: The optimum conditions of ultrasonic extraction was ethanol concentration 85%, ultrasonic time 40 min, extraction power 126 W and the ration of solid-liquid 1:30 g/mL, which the highest yield was 1.327%.2. Research on Purification: In order to get triterpenoids with high purity, HPD500 macroporous resin was selected from seven macroporous resin namely AB-8、D101、S-8、HPD100 、 HPD300 、 HPD500 、 D301 for purification by adsorption and desorption experiments. The optimum conditions of purification were: the solution pH 6, solution concentration 1 mg/mL, ethanol concentration 70%, flow rate 1 mL/min, the purity of the triterpenoids increased from 26.8 % to 40.4 % after purification.3. Physiological activities:(1) Research on antioxidant in vitro: this test has researched antioxidant of crude ethanol extract(CEE), petroleum ether fraction(PEF), ethyl acetate fraction(EAF) and n-butanol fraction(NBF) of GPM from six aspects in(DPPH?, ABTS+?, ?OH, ?O2–,) scavenging capacity assay, reducing capacity assay, and total antioxidant assay. The results suggested that the order of antioxidant capacity are: EAF>NBF>CEE>PEF, and the antioxidant have dose-effect.(2) MTT assay: Research on cell proliferation of human colon cancer Caco-2 cells by treated with CEE, PEF, EAF, and NBF at different concentrations. The results indicated that the order of human colon cancer Caco-2 cells proliferation are: EAF>NBF>CEE>PEF, and the human colon cancer Caco-2 cell proliferation of GPM extracts have dose-effect.The research findings all indicated that EAF have the best antioxidant abilities and cells proliferation.