Preparation Of The Collagen-PAPBA Nanoparticles Encapsulating Doxorubicin And It’s Anticancer Efficacy Study In Ovarian Cancer

Objective The study was designed to prepare the collagen-PAPBA nanoparticles encapsulating doxorubicin and it’s anticancer efficacy in ovarian cancer.Methods1.We first make the preparation of collagen-PAPBA nanoparticles encapsulating doxorubicin. Then, dynamic light scattering and transmission electron microscopy have been used to observe the particle size and morphology of collagen-PAPBA nanoparticles.2.Doxorubicin has been loaded on the nanoparticles.3.Dynamic light scattering and transmission electron microscopy have been appicated to observe the preparation of drug loaded nanoparticle size distribution and morphology.4.We also study the determination of drug loaded nanoparticles and entrapment efficiency.5.In vitro release of the methylprednisolone and the cytotoxicity of the doxorubicin loaded nanoparticles against A2780 cells were evaluated.6.In our study, we establish a model of ovarian cancer xenograft tumor in rats, and observe the anticancer efficacy of collagen-PAPBA nanoparticles encapsulating doxorubicin.Results1.We have successfully made the preparation of the collagen-PAPBA nanoparticles encapsulating doxorubicin. The average hydrodynamic diameter of collagen-PAPBA nanoparticles were measured by dynamic light scattering(DLS) as about79 nm, with homogeneous distribution of size. Transmission electron microscopy(TEM) was used to investigated the nanoparticles, while the nanoparticles were dispersed well, spherical, about 75 nm and uniform in size.2.Collagen-PAPBAnanoparticles showed no sense to PH. The nanoparticles were associated with the temperature and the concentration of glucose. The average hydrodynamic diameter of doxorubicin loaded collagen-PAPBA nanoparticles was measured by DLS as about 81.3nm, with homogeneous distribution of size. TEM was used to observe the drug loaded nanoparticles as being spherical and well dispersed in size distribution, mainly in 79 nm.3.The amount of DOX loaded on the nanoparticles was 10%,while the encapsulating efficiency was 97%.4.The result of the releasing test showed that the drug loaded nanoparticles, as carriers for DOX, had the characteristics of good sustained release effect and could have tumor selective release.5.Cell toxicity experiment showed that the blank collagen-PAPBA nanoparticles have no cytotoxicity to A2780 cells, and that the drug loaded nanoparticles have good sustained release function and may have potential reducing toxic side effects.6.At the same doses, the drug loaded nanoparticles have superior inhibitory effect on the growth of human ovarian cancer xenograft tumor.Conclusions We have successfully prepared Collagen-PAPBA nanoparticles encapsulating doxorubicin. Collagen-PAPBAnanoparticles encapsulating doxorubicin have supurior anti-ovariancancer compared with free nanoparticles.