Regulation Of Mesenchymal Stem Cells On Notch Signaling Pathway In Asthmatic Rats

Objective To investigate asthmatic rats lymph, blood and lung tissue changes of signaling Notch signal pathways, and the study of human placental mesenchymal stem cells impact on the Notch signaling pathway in asthmatic rats.Methods Collagenase enzyme digestion in vitro and amplified human placenta mesenchymal stem cells cultured in vitro passaged cell count, growth curve, cell surface markers and osteogenic means induced to identify placenta-derived mesenchymal stem cells; established asthma large mouse model; conduct human placenta mesenchymal stem cell transplantation for treatment:the 30 male SD rats were randomly divided into three groups, each 10:(1) normal control group (2) asthma group (3) human placental mesenchymal mesenchymal stem cell transplantation group; rats after the last challenge, collect rat lung tissue, blood, lymph, were extracted from the blood, lymph mononuclear cells (MNC), using real-time quantitative PCR receptor Notch1, Notch2, Notch3 and ligand Jagged1, Delta4 expression.Result 1.hPMSCs isolation, culture, cell surface markers and a number of differentiation:using enzyme digestion were isolated and cultured human placental MSCs; increased with the number of passages, human placental MSCs proliferation diminished capacity; flow cytometry CD73 expression, CD90, CD 105, low expression of CD34, CD45, HLA-DR; In vitro experiments showed that human placental MSCs to differentiate into multiple differentiation may osteoblasts and fat cells.2.24h collected after the last shot of lung tissue, asthma group Notch1, express Notch2, JaggedlmRNA significantly higher than hPMSCs transplantation group and the normal group (P <0.05), Notch3, Delta4mRNA expression compared hPMSCs significantly reduced transplant group and the normal group expression (P<0.05), hPMSCs transplantation group and the normal group was insignificant difference.3.24h after the last challenge to collect blood, blood mononuclear cells express asthma group Notch1, Notch2, Jagged1mRNA significantly higher than hPMSCs transplantation group and the normal group (P<0.05), the expression of Notch3, Delta4mRNA than hPMSCs transplantation group and the normal group expression was significantly lower (P<0.05), hPMSCs transplantation group and the normal group meaningless expression differences.4.24h after the last challenge to collect lymph, blood mononuclear cells in the asthmatic group Notchl, Notch2, Jaggedlm-RNA expression was significantly higher than hPMSCs transplantation group and the normal group (P<0.05), the expression of Notch3, Delta4mRNA transplantation group than hPMSCs and the normal group was significantly lower (P<0.05), hPMSCs transplantation group and the normal group meaningless expression differences.5.Western blot analysis of lung tissue in asthma group Notchl, Notch2, Jaggedl protein expression was significantly higher than hPMSCs transplantation group and the normal group (P <0.05), Notch3, Delta4 protein compared hPMSCs transplantation group and the normal group was significantly lower (P<0.05), hPMSCs transplantation group and the normal group was insignificant difference.Conclusion 1.By enzyme digestion successfully isolated and cultured human placental passage mesenchymal stem cells, suggesting that the expression of stem cell phenotype, and the osteogenic differentiation of fat cells.2.Asthma group, the presence of blood and lymph mononuclear cells Notchl, express Notch2, Jaggedl, Delta4 increase, decrease Notch3, Delta4 expression, this change presumably immune response of asthma.3.Human placental mesenchymal stem cells can regulate the expression of the Notch signaling pathway to inhibit inflammation of the airways in asthmatic rats.