The Study Of Porphyromonas Gingivalis And Its Virulence Factor Gingipain K During Orthodontic Treatment

Objective:In the progress of orthodontic treatment, many past studies have showed that various kinds of factors all can lead to gingivitis inflammation, there is closely relationship between Porphyromonas gingivalis and periodontal disease or teenagers periodontitis.But there has rarely discuss between Porphyromonas gingivalis and its virulence factors Gingipain K adhesion element and gingivitis inflammation in the course of orthodontic treatment.This study had applied 16SrDNA PCR skill to detect 45 orthodontics treatment patients’Porphyromonas gingivalis or its virulence factors Gingipain K adhesion element in gingival crevicular fluid, to discuss the relevance between them and the gingivitis inflammation in the process of orthodontic treatment, and to analyse its impact in the progress of orthodontic gingivitis happening.Methods:To establish 16SrDNA PCR which is a kind of skill to check out Porphyromonas gingivalis or its virulence factors Gingipain K adhesion element, make use of Porphyromonas gingivalis to detect its sensitivity and specificity.Totally 45 orthodontic healthy teenagers were randomly chosen from the orthodontic:Before orthodontic treatment, the first month after treatment or the second month and third month after treatment or its virulence factors Gingipain K adhesion element which are in gingival crevicular fluid, to analyse the relationship between it and GI.Results:Porphyromonas gingivalis before treatment and the first month after treatment have outstanding difference (P< 0.05).There are remarkable difference among before treatment, the second month after treatment and the third month after treatment (P<0.01).The first month after treatment and the second month after treatment are diversity.Gingipain K before treatment have no difference between the first month after treatment and the sixth month after treatment, but have discrepancy between the second month after treatment and the third month after treatment (P< 0.05).Since the third month after treatment, the ratio of porphyromonas gingivalis and gingipain K had declined, in the sixth month after treatment it came close to before treatment.Conclusion:1. Fixed appliance which in orthodontic treatment can give rise to gingivitis inflammation, which can also lead to GI growing.2. Porphyromonas gingivalis and Gingipain K have been closely bounded up with the pathogenic bacteria, which can also play a part in the gingivitis inflammation occured in the course of orthodontic treatment.3. Gingipain K has been closely related to the GI growing, which can play a part role in the gingivitis inflammation happened in the course of orthodontic treatment.