Effect Of C3aRA And C5aRA On The Expression Of IL-6, MCP-1 And Proliferation In Human Mesangial Cells Stimulated By IgA1

ObjectivesIgA1 was used to stimulated human mesangial cells(HMCs) in this study to investigate the effects of C3aRA and C5aRA on the expression of IL-6,MCP-1 and proliferation. Methods1. MTT assay was used to detect the effect of IgA1 on the proliferation in HMCs after different concentrations and incubation time;2. The HMCs were divided into four groups: control group, IgA1 group, IgA1 + C3aRA group and IgA1 + C5aRA group, MTT assay was used to detect the effect of C3aRA, C5aRA on the proliferation in HMCs treated with IgA1; fluorescence quantitative PCR(RT-qPCR) and Western blot was used to detect the expression of C3aR, C5aR mRNA;3. The HMCs were divided into two groups which were further divided into four subgroups: control group, C3 a group, IgA1+C3a group and IgA1+C3a+C3aRA group; control group, C5 a group, IgA1+C5a group and IgA1+C5a+C5aRA group. RT-q PCR and Western blot was used to detect mRNA and protein expression of IL-6 and MCP-1. Results1. MTT results showed that different concentrations(10μg / mL-100 mg / mL)of IgA1 can stimulate the proliferation of HMCs; 100μg / mL IgA1 stimulus HMCs 24 h can significantly enhanced the proliferation of HMCs.2. MTT results showed that compared with control group, IgA1 group showed significant proliferation activity, while after intervention with C3aRA and C5aRA, C3aRA group and C5aRA group showed partly inhibited proliferation; RT-qPCR and Western blot results showed that compared with the control group, HMCs showed significantly up-regulation of C3aR and C5aR expression induced by IgA1, C3aRA and C5aRA can partly blocked the expression of C3aR and C5aR, while remain higher than the control group.3. RT-qPCR and Western blot results showed that IgA1,C3 a and C5 a stimulation alone could up-regulate the expression of IL-6 and MCP-1, when IgA1 stimulated HMCs together with C3 a or C5 a, it could significantly increase the expression of IL-6 and MCP-1, which could be partly blocked by C3aRA and C5aRA.. Conclusions1. IgA1 can stimulated the proliferation of HMCs, and could be partly blocked by C3aRA and C5aRA.2. HMCs stimulated by IgA1 can increase the expression of C3aR and C5aR, and C3aRA, C5aRA can reduce the expression of C3aR, C5 a R.3. C3a and C5a can respectively binding up-regulated C3aR, C5aR and increase the expression of IL-6, MCP-1 in HMCs stimulated by IgA1, C3aRA and C5aRA can reduce the expression of IL-6, MCP-1.