Electroacupuncture On Type Ⅱ Diabetic Peripheral Neuropathic Pain Effect Of JAK2/STAT3 Signaling Pathway In Rat Spinal Cord

Objective:Observation of electro-acupuncture on rats with type II diabetic peripheral neuralgia pain behavior and spinal cord p-JAK2 and p-STAT₃ expression of,explore the mechanism of electro-acupuncture in treatment of diabetic neuropathic pain.Rats regularly bundled control group and model groupMethods:Fourty male Sprague-Dawley rats aging 6 week,8 randomly selected as control group.The remaining 32 rats prepared with type 2 diabetes peripheral neuropathy model according to the literature method and molded 24.Model rats were randomly divided into three groups（n=8）:model group,electropuncture group and acupuncture group.Control group and model group were regularly tied up,electropuncture group and acupuncture group select "jump ring" and "Yanglingquan" treatment for 3 weeks.On day 0（T₀）,day 56（T₁）,day 70（T₂）measurement of fasting glucose,insulin concentration and mechanical paw withdrawal response threshold（PWT）,in the first96 days（T₃）measured only PWT.Upon completion of all measurements T₃ in taking chloral hydrate anesthesia lumbar spinal cord was observed by Western blotting assessment of spinal p-JAK2 p-STAT₃ expression.Result:（1）The difference in weight of rats in each group was not statistically significant at T₀（P>0.05）;Weight was significantly higher in model group,electropuncture group and acupuncture group at T₁,the difference was statistically significant（P < 0.05）,Weight was significantly higher in model group,electropuncture group and acupuncture group at T₂,the difference was statistically significant（P <0.05）.（2）Blood glucose level of rats in each group no significant difference at T₀（P>0.05）;Blood glucose level of rats in each group no significant difference at T₁（P> 0.05）;Blood glucose level was significantly higher in model group,electropuncture group and acupuncture group,the difference was statistically significant at T₂（P<0.05）.（3）Differences insulin concentrations and insulin sensitivity coefficient of the rats no significant difference at T₀（P>0.05）;model group,electropuncture group and acupuncture group insulin concentrations and insulin sensitivity index was significantly higher,the difference was statistically significant at T₁（P<0.05）;model group,electropuncture group and acupuncture group insulin concentrations and insulin sensitivity index was significantly higher,the difference was statistically significant at T₂（P<0.05）.（4）PWT value was no significant difference between the groups of rats at T₀（P>0.05）;PWT values still no statistical significance of the rats（P>0.05）;PWT value of the control group was significantly higher than model group,electropuncture group and acupuncture group,the difference was statistically significant at T₂（P<0.05）;At T₃,Comparison of T₂,PWT and electropuncture group was significantly higher（P<0.05）than the acupuncture group.PWT model group was no significant difference at compared with T₂（P>0.05）.（5）Western blot results suggest that the control group p-JAK2 and p-STAT₃ was the lowest,followed by electropuncture group group,cupuncture group was more than the amount of the aforementioned groups,but the highest expression in the model group.Conclusion:Eectropuncture may be through inhibition of the spinal cord JAK2 / STAT₃ signaling pathway activation and inhibition of type 2 diabetes alleviate neuropathic pain.