The Preparation And Disinfection Performance Of Three Dimensional Ordered Mesoporous Carbon Immobilized With Lysozyme

Water, one of the most precious natural resources on earth, is being contaminated by various pollutions created by human activities. Among them, microbial contamination of water causes a major menace to public health and aquatic species. Especially pathogenic bacteria are responsible for waterborne diseases and have detrimental effect on human health. Although the traditional disinfection technology can effectively kill pathogenic bacteria, there exists the formation of harmful disinfection byproducts, the high cost. In order to solve these problems, more and more researchers begin to pay close attention to some low-cost and natural disinfectannt, such as chitosan, lysozyme, etc. Therefore, aims to develop a safe, reliable and effective disinfection treatment technology to replace the traditional disinfection technology, this work successfully synthesized a novel antibacterial material.In this study, an effective water disinfection agent was proposed based the three dimensional ordered mesoporous carbon (3DOm C) with lysozyme impregnated by covalent cross linking. In the synthesis of 3DOm C, ordered silica nanoparticles are used a template, furfuryl alcohol as carbon source. Critical immobilization parameters, such as lysozyme initial concentration, pH value, time, temperature, were optimized to keep the activity of the immobilized lysozyme at a high level. And, the disinfecting materials have been characterized by transmission electron microscopy, Fourier infrared spectrum, and small Angle X-ray diffraction spectrum. The antibacterial performance of disinfecting materials was specifically investigated based on the disinfection efficacy of Staphylococcus aureus in water. Also, the effect of pH on the bacteria removal rate and the reusability of disinfecting material were discussed.The result revealed that 3DOm C posses highly ordered mesoporous pore, pore size is adjustable, and has a larger specific surface area and pore volume. Although the pore structure was damaged due to the oxidation treatment of nitric acid, the main three dimensional network structures is still preserved, and carboxyl functional groups were successfully introduced into 3DOm C; The characterization results suggest that lysozyme molecules are firmly immobilized on the carrier, and the secondary structure of lysozyme does not change. The results revealed that the immobilization capacity and relative activity of the immobilized lysozyme were 842 mg/g carrier and 81%, respectively, under the optimal immobilization conditions, lysozyme concentration of 2 mg/mL, pH= 10, time is 9 h, temperature is 25℃. But in the sterilization experiments, we found that the largest bacteria removal rate (96.5%) is achieved when the lysozyme concentration of 3 mg/mL, pH can affect disinfection efficiency, when the pH is 5,98.1% of the bacteria can be removed within 2 h. This disinfection material also has lasting disinfection effect; the antibacterial behavior of this disinfection material is an integration of the premier adsorption by 3DOm C and the hydrolysis of lysozyme.