Study On The Inhibition And Proliferation Of BGC823 Gastric Cancer Cell Line And HepG2 Hepatocellular Carcinoma Solid Transplantation Tumor With Black Garlic Extract

The purpose:Observe black garlic extract (Aged Black Garlic Extract) on BGC823 gastric cancer cell lines and xenografts solid hepatoma Hep inhibit proliferation and to explore the mechanisms that may exist. And to further explore the black garlic extract to enhance and improve the mechanism of immunity. For Integrative develop innovative cancer treatments and therapies to provide relevant research basisMethods:In vitro experiments designed using conventional culture BGC823 gastric cancer cells, in logarithmic growth phase cells with different concentrations of black garlic extract to intervene, MTT assay black garlic extract on the growth inhibition of gastric cancer cells BGC823; cells were observed under an inverted microscope apoptotic morphology; flow cytometry cell cycle; animal experiments to establish solid hepatoma Hep xenograft mouse model for intervention in a certain time after treatment, the mice were killed by cervical off, and the inhibition rate of tumor weight was measured in light tumor tissue morphology were observed; the determination of tumor apoptosis index; spleen and thymus of mice measured after administration.Results:1, black garlic extract on gastric cancer cell line BGC823 significantly inhibit growth and induce apoptosis (P<0.05), and have the time, the concentration of the dose-effect relationship.2, black garlic extract on ascites tumor bearing mice and tumor weight inhibition rate has a significant effect (P<0.05), black garlic extract on Hep liver xenografts entity inhibitory effect on tumor-bearing and due to a small change mouse spleen and thymus have significant effect (P<0.05).Conclusion:BGC823 black garlic extract on the growth inhibition of gastric cancer cells:black garlic extract of Hep liver transplanted solid tumor suppressor role in proliferation and improve immunity.