The Correlated Expression Analysis Of MiRNA And MRNA In The Acute Aortic Dissection Of Stanford A Type

Objective:Differential expression of miRNA and mRNA in the media of vessels wall of acute A type Stanford aortic dissection were selected.The expression profiles of mi RNA and mRNA was Constructed,futhermore, the signaling pathway and molecular mechanism was explored for the disease.Methods:To select 14 cases of Stanford type A aortic dissection the media of vessels wall involving the aortic membrane(case group,Group A),14 cases of the membrane normal aortic wall tissue in distal dissection(self control,Group B),10 cases of Ascending aorta tissue of voluntary tissue donor for exclusion of aortic disease( control group).The sex of all samples were male Chinese.Through, the differential expression of miRNA and mRNA was select by the Agilent gene chip technology to constructe the expression profile, Possible signaling pathway and molecular regulation mechanism was analyzed by bioinformatics analysis.Results: 1.Compared with group C, there are 87 mi RNAs increasing expression and 71 miRNAs decreasing expression in group A;2.The results showed that the difference of hsa-miR-21-3p, hsa-miR-181a-5p, hsa-mi R-181b-5p was significantly. SV2 C, HTR6, MAST1 and other 96 gene were predicted to be the target genes;3.The correlated expression analysis of miRNA and mRNA were constructed;4.The results showed that the extracellular matrix receptor, focal adhesion plaque and NF-kB and other 8 signal pathway may be related to the onset and development of the disease.Conclusion: 1.Hsa-miR-21-3p may regulate to target gene SV2 C in vascular smooth muscle cell migration, phenotype transformation and extracellular matrix homeostasis, and regulate to target gene ACTN2 in skeleton protein synthesis and vascular fibrosis to promote the incidence of aortic dissection.2.Hsa-miR-181a-5p may participate in cAMP signaling, calcium signaling pathway and NF-kB pathway through the regulation of target gene HTR6, and participate in vascular inflammation and atherosclerosis in the pathogenesis of aortic dissection.Hsa-miR-181b-5p may regulate in vascular smooth muscle cell migration, phenotype transition and extracellular matrix homeostasis by regulating target gene SV2 C in the pathogenesis of aortic dissection.3.Differential expression of miRNA and mRNA complex regulatory network may be complex to control the occurrence and development of diseases.