The Effect Of S100A4 Gene Expression Silence On Apoptosis And Invasion In CNE2 Cell

Objective:To observe the effect of S100A4 gene expression silence in CNE2 cell with RNA interference technique and to explore the affection on invasion ability, apoptosis,P53 and E-cadherin protein expression in CNE2 cell after S100A4 gene expression was decreased,disscuss the function and mechanism of S100A4 gene.Methods:S100A4siRNA cells were successfully transfected in CNE2 by liposome method, We transfect siRNA-S100A4-CNE2 cells to the experimental group, than NCsiRNA-S100A4-CNE2 cell line and non transfected CNE2 cells were divided into the control group. Western blotting and Real-time PCR were used to detect the expression level of S100A4 gene protein and mRNA in each group cells, The apoptosis and invasive ability of each group cells was analyzed by flow cytometry and transwell assay, Inspecting the expression level of P53 and E-cadherin protein in three groups of cells by western blotting. Results:1、Western blotting shows that the expression of S100A4 protein in siRNA-S100A4-CNE2 cells is decreased.2 、 Real-time PCR test results show that the expression of S100A4 mRNA in siRNA-S100A4-CNE2 cell line is decreased.3、Flow cytometry shows that the number of apoptotic cells in the siRNA-S100A4-CNE2 cell line is significantly increased.4 、 Transwell test shows that the number of acrossing the cell membrane in the siRNA-S100A4-CNE2 cell line is significantly reduced.5 、 Western blotting shows that the expression of P53 and Ecadherin protein in siRNA-S100A4-CNE2 cell line is increased.Conclusion:1、S100A4siRNA transfection could effectively silence the gene in CNE2 cell, promote the apoptosis of CNE2 cells, and inhibit the invasion of CNE2 cells.2、The expression regulation of S100A4 gene in nasopharyngeal carcinoma CNE2 cells can promote the expression of P53 and E-cadherin protein.